Journal article
A rapid allele-specific assay for HLA-A*32:01 to identify patients at risk for vancomycin-induced Drug Reaction with Eosinophilia and systemic symptoms
The Journal of Molecular Diagnostics, Vol.21(5), pp.782-789
2019
Abstract
Human leukocyte antigen (HLA) alleles have been implicated as risk factors for immune-mediated adverse drug reactions. We recently reported a strong association between HLA-A*32:01 and vancomycin-induced drug reaction with eosinophilia and systemic symptoms (DRESS). Identification of individuals with the risk allele prior to or shortly after the initiation of vancomycin therapy is of great clinical importance to prevent morbidity and mortality, improve drug safety and antibiotic treatment options. A prerequisite to the success of a pharmacogenetic screening tests is the development of simple, robust, cost-effective single HLA allele test that can be implemented in routine diagnostic laboratories. In this study, we developed a simple, real-time allele-specific PCR for typing the HLA-A*32:01 allele. Four-hundred and fifty-eight DNA samples including thirty HLA-A*32:01-positive samples were typed by allele-specific PCR. Compared to ASHI accredited sequence-based high-resolution, full allelic HLA typing, this assay demonstrates 100% accuracy, sensitivity of 100% (95% CI: 88.43% to 100%) and specificity of 100% (95% CI: 99.14% to 100%). The lowest limit of detection of this assay using the Power Up SYBR Green is 10 ng of template DNA. The assay demonstrates a sensitivity and specificity to differentiate HLA-A*32:01 allele from closely related non-HLA-A*32 alleles and may be used in clinical settings to identify individuals with the risk allele prior or during the course of vancomycin therapy…
Details
- Title
- A rapid allele-specific assay for HLA-A*32:01 to identify patients at risk for vancomycin-induced Drug Reaction with Eosinophilia and systemic symptoms
- Authors/Creators
- F.X. Rwandamuriye (Author/Creator)A. Chopra (Author/Creator)K.C. Konvinse (Author/Creator)L. Choo (Author/Creator)J.A. Trubiano (Author/Creator)C.M. Shaffer (Author/Creator)M. Watson (Author/Creator)S.A. Mallal (Author/Creator)E.J. Phillips (Author/Creator)
- Publication Details
- The Journal of Molecular Diagnostics, Vol.21(5), pp.782-789
- Publisher
- Elsevier Inc.
- Identifiers
- 991005543609507891
- Copyright
- © 2019 Elsevier Inc. on behalf of the American Society for Investigative Pathology and the Association for Molecular Pathology
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 1 Clinical & Life Sciences
- 1.265 Dermatology - Skin Allergies
- 1.265.1140 Drug Hypersensitivity
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine