Journal article
A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles
International Journal of Laboratory Hematology, Vol.31(4), pp.430-439
2009
Abstract
Platelet activation occurs in a variety of clinical situations in which it directly contributes to the pathology. This study reports a simple flow cytometric assay for platelet activation which measures platelet-derived microparticles, activated platelets and platelet–monocyte complexes. Pre- and post analytical conditions were investigated and optimized and a normal range established on 20 healthy controls. Twenty patients pre- and post percutaneous coronary intervention (PCI) were tested with the technique. Soluble activation markers sCD40 ligand and sP-selectin and plasma phospholipid levels were measured in both groups. There was a significant increase in activated platelets and platelet–monocyte complexes between normal and pre-PCI (P = 0.005 and 0.0275, respectively) suggesting an activated state. There was a significant fall in activated platelets post-PCI (P = 0.0027) which was mirrored by a fall in soluble CD40 ligand, soluble P-selectin and plasma phospholipid levels (P = 0.0066, <0.0001 and 0.0032, respectively) consistent with antiplatelet therapy administered during the process. This is a reliable and rapid method for the assessment of ex vivo platelet activation which may be an aid in diagnosis and help guide therapy for patients with thrombotic disease.
Details
- Title
- A rapid flow cytometric technique for the detection of platelet-monocyte complexes, activated platelets and platelet-derived microparticles
- Authors/Creators
- L. Pearson (Author/Creator) - Royal Perth HospitalJ. Thom (Author/Creator) - Royal Perth HospitalM. Adams (Author/Creator) - Curtin UniversityR. Oostryck (Author/Creator) - Curtin UniversityR. Krueger (Author/Creator) - Royal Perth HospitalG. Yong (Author/Creator) - Royal Perth HospitalR. Baker (Author/Creator) - Royal Perth Hospital
- Publication Details
- International Journal of Laboratory Hematology, Vol.31(4), pp.430-439
- Publisher
- Wiley
- Identifiers
- 991005542366307891
- Copyright
- © 1999 - 2017 John Wiley & Sons, Inc.
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.935 Adhesion Molecules
- Web Of Science research areas
- Hematology
- ESI research areas
- Clinical Medicine