Journal article
A role for Huntington disease protein in dendritic RNA granules
Journal of Biological Chemistry, Vol.285(17), pp.13142-13153
2010
Abstract
Regulated transport and local translation of mRNA in neurons are critical for modulating synaptic strength, maintaining proper neural circuitry, and establishing long term memory. Neuronal RNA granules are ribonucleoprotein particles that serve to transport mRNA along microtubules and control local protein synthesis in response to synaptic activity. Studies suggest that neuronal RNA granules share similar structures and functions with somatic P-bodies. We recently reported that the Huntington disease protein huntingtin (Htt) associates with Argonaute (Ago) and localizes to cytoplasmic P-bodies, which serve as sites of mRNA storage, degradation, and small RNA-mediated gene silencing. Here we report that wild-type Htt associates with Ago2 and components of neuronal granules and co-traffics with mRNA in dendrites. Htt was found to co-localize with RNA containing the 3′-untranslated region sequence of known dendritically targeted mRNAs. Knockdown of Htt in neurons caused altered localization of mRNA. When tethered to a reporter construct, Htt down-regulated reporter gene expression in a manner dependent on Ago2, suggesting that Htt may function to repress translation of mRNAs during transport in neuronal granules.
Details
- Title
- A role for Huntington disease protein in dendritic RNA granules
- Authors/Creators
- J.N. Savas (Author/Creator) - New York UniversityB. Ma (Author/Creator) - New York UniversityK. Deinhardt (Author/Creator) - New York UniversityB.P. Culver (Author/Creator) - New York UniversityS. Restituito (Author/Creator) - New York UniversityL. Wu (Author/Creator) - New York UniversityJ.G. Belasco (Author/Creator) - New York UniversityM.V. Chao (Author/Creator) - New York UniversityN. Tanese (Author/Creator) - New York University
- Publication Details
- Journal of Biological Chemistry, Vol.285(17), pp.13142-13153
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Identifiers
- 991005546031807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.52 Neurodegenerative Diseases
- 1.52.951 Huntington's and Ataxias
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry