Journal article
A simple method for cloning Giardia duodenalis from cultures and fecal samples
Journal of Parasitology, Vol.77(4), pp.627-631
1991
Abstract
Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.
Details
- Title
- A simple method for cloning Giardia duodenalis from cultures and fecal samples
- Authors/Creators
- N. Binz (Author/Creator)R.C.A. Thompson (Author/Creator)B.P. Meloni (Author/Creator)A.J. Lymbery (Author/Creator)
- Publication Details
- Journal of Parasitology, Vol.77(4), pp.627-631
- Publisher
- American Society of Parasitologists
- Identifiers
- 991005540997107891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
Metrics
305 File views/ downloads
45 Record Views