Journal article
A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification
Biotechnology Letters, Vol.22(2), pp.105-108
2000
Abstract
A simple and economical method is described that allows rapid detection of laccase activity in chromatography column fractions during enzyme purification. Aliquots of column eluants are applied to filter paper coated with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) containing a numbered grid, and incubated at ambient temperature for 20 min. Indications of enzyme activity are simply observed by a colour change. This method avoids having to manually assay each fraction of a chromatographic run for enzyme activity.
Details
- Title
- A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification
- Authors/Creators
- R.F.H. Dekker (Author/Creator) - Murdoch UniversityK.Y. Ling (Author/Creator) - Universidade Estadual de LondrinaA.M. Barbosa (Author/Creator) - Universidade Estadual de Londrina
- Publication Details
- Biotechnology Letters, Vol.22(2), pp.105-108
- Publisher
- Springer Netherlands
- Identifiers
- 991005541539307891
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
153 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.87 Paper & Wood Materials Science
- 3.87.946 Ligninolytic Enzymes
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- ESI research areas
- Biology & Biochemistry