A sodium transporter HvHKT1;1 confers salt tolerance in barley via regulating tissue and cell ion homeostasis

Y. Han; S. Yin; L. Huang; X. Wu; J. Zeng; X. Liu; L. Qiu; R. Munns; Z-H Chen; G. Zhang

doi:10.1093/pcp/pcy116

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A sodium transporter HvHKT1;1 confers salt tolerance in barley via regulating tissue and cell ion homeostasis

Journal article

Peer reviewed

A sodium transporter HvHKT1;1 confers salt tolerance in barley via regulating tissue and cell ion homeostasis

Y. Han, S. Yin, L. Huang, X. Wu, J. Zeng, X. Liu, L. Qiu, R. Munns, Z-H Chen and G. Zhang

Plant and Cell Physiology, Vol.59(10), pp.1976-1989

2018

DOI: https://doi.org/10.1093/pcp/pcy116

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Abstract

Our previous studies showed that high salt tolerance in Tibetan wild barley accessions was associated with HvHKT1;1, a member of the high-affinity potassium transporter family. However, molecular mechanisms of HvHKT1;1 for salt tolerance and its roles in K+/Na+ homeostasis remain to be elucidated. Functional characterization of HvHKT1;1 was conducted in the present study. NaCl-induced transcripts of HvHKT1;1 were significantly higher in the roots of Tibetan wild barley XZ16 relative to other genotypes, being closely associated with its higher biomass and lower tissue Na+ content under salt stress. Heterologous expression of HvHKT1;1 in Saccharomyces cerevisiae (yeast) and Xenopus laevis oocytes showed that HvHKT1;1 had higher selectivity for Na+ over K+ and other monovalent cations. HvHKT1;1 was found to be localized at the cell plasma membrane of root stele and epidermis. Knock-down of HvHKT1;1 in barley led to higher Na+ accumulation in both roots and leaves, while overexpression of HvHKT1;1 in salt-sensitive Arabidopsis hkt1-4 and sos1-12 loss-of-function lines resulted in significantly less shoot and root Na+ accumulation. Additionally, microelectrode ion flux measurements and root elongation assay revealed that the transgenic Arabidopsis plants exhibited a remarkable capacity for regulation of Na+, K+, Ca2+ and H+ homeostasis under salt stress. These results indicate that HvHKT1;1 is critical in radial root Na+ transport, which eventually reduces shoot Na+ accumulation. Additionally, HvHKT1;1 may be indirectly involved in retention of K+ and Ca2+ in root cells, which also improves plant salt tolerance.

Details

Title: A sodium transporter HvHKT1;1 confers salt tolerance in barley via regulating tissue and cell ion homeostasis
Authors/Creators: Y. Han (Author/Creator) - Zhejiang University
S. Yin (Author/Creator) - Zhejiang University
L. Huang (Author/Creator) - Zhejiang University
X. Wu (Author/Creator)
J. Zeng (Author/Creator) - Zhejiang University
X. Liu (Author/Creator) - Western Sydney University
L. Qiu (Author/Creator) - Zhejiang University
R. Munns (Author/Creator) - ARC Centre of Excellence in Plant Energy Biology
Z-H Chen (Author/Creator) - Zhejiang University
G. Zhang (Author/Creator) - Zhejiang University
Publication Details: Plant and Cell Physiology, Vol.59(10), pp.1976-1989
Publisher: Oxford University Press
Identifiers: 991005544697007891
Copyright: 2018 The Author(s).
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.49 Plant Stress Responses
Web Of Science research areas: Cell Biology; Plant Sciences
ESI research areas: Plant & Animal Science