Journal article
A standard panel of microsatellites for Asian seabass(Lates calcarifer)
Animal Genetics, Vol.41(2), pp.208-212
2010
Abstract
Microsatellites are the most popular markers for parentage assignment and population genetic studies. To meet the demand for international comparability for genetic studies of Asian seabass, a standard panel of 28 microsatellites has been selected and characterized using the DNA of 24 individuals from Thailand, Malaysia, Indonesia and Australia. The average allele number of these markers was 10.82 +/- 0.71 (range: 6-19), and the expected heterozygosity averaged 0.76 +/- 0.02 (range: 0.63-1.00). All microsatellites showed Mendelian inheritance. In addition, eight standard size controls have been developed by cloning a set of microsatellite alleles into a pGEM-T vector to calibrate allele sizes determined by different laboratories, and are available upon request. Seven multiplex PCRs, each amplifying 3-5 markers, were optimized to accurately and rapidly genotype microsatellites. Parentage assignment using 10 microsatellites in two crosses (10 x 10 and 20 x 20) demonstrated a high power of these markers for revealing parent-sibling connections. This standard set of microsatellites will standardize genetic diversity studies of Asian seabass, and the multiplex PCR sets will facilitate parentage assignment.
Details
- Title
- A standard panel of microsatellites for Asian seabass(Lates calcarifer)
- Authors/Creators
- Z.Y. Zhu (Author/Creator)C.M. Wang (Author/Creator)L.C. Lo (Author/Creator)G. Lin (Author/Creator)F. Feng (Author/Creator)J. Tan (Author/Creator)R. Chou (Author/Creator)H.S. Lim (Author/Creator)L. Orbán (Author/Creator)G.H. Yue (Author/Creator)
- Publication Details
- Animal Genetics, Vol.41(2), pp.208-212
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005543495007891
- Copyright
- © 2009 The Authors, Journal compilation © 2009 Stichting International Foundation for Animal Genetics
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.64 Phylogenetics & Genomics
- 3.64.71 Genetic Diversity
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
- Genetics & Heredity
- ESI research areas
- Plant & Animal Science