A technique for the quantification of human and non-human mammalian mitochondrial DNA copy number in forensic and other mixtures

S.S. Tobe; A.M.T. Linacre

doi:10.1016/j.fsigen.2008.03.002

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A technique for the quantification of human and non-human mammalian mitochondrial DNA copy number in forensic and other mixtures

Journal article

Peer reviewed

A technique for the quantification of human and non-human mammalian mitochondrial DNA copy number in forensic and other mixtures

S.S. Tobe and A.M.T. Linacre

Forensic Science International: Genetics, Vol.2(4), pp.249-256

2008

DOI: https://doi.org/10.1016/j.fsigen.2008.03.002

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Abstract

The number of mitochondria per cell varies by cell type and the number of mitochondrial DNA (mtDNA) genomes varies per mitochondrion. Biological samples from unknown species are encountered frequently in forensic science investigations and are often contaminated with human mtDNA making analysis difficult. Currently, no techniques to quantify non-human mtDNA are available. We report on a method to accurately quantify, sensitive to 100 copies (1.7 fg), mtDNA from human and non-human sources when present as a mixture. The test developed uses the cytochrome b (cytb) and the ribosomal 12S genes on the mitochondrial genome. Universal and human specific fragments of similar size are amplified and quantified using SYBR Green. We validate the test with 24 human samples and 27 non-human mammalian samples. The human fraction of a sample can then be subtracted from the universal fraction for an accurate estimation of non-human mtDNA copy number.

Details

Title: A technique for the quantification of human and non-human mammalian mitochondrial DNA copy number in forensic and other mixtures
Authors/Creators: S.S. Tobe (Author/Creator) - International Union of Pure and Applied Chemistry
A.M.T. Linacre (Author/Creator)
Publication Details: Forensic Science International: Genetics, Vol.2(4), pp.249-256
Publisher: Elsevier BV
Identifiers: 991005540023307891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 2 Chemistry; 2.244 Chemometrics; 2.244.2133 Molecular Authentication
Web Of Science research areas: Genetics & Heredity; Medicine, Legal
ESI research areas: Molecular Biology & Genetics