Journal article
A thin-layer multistrip agarose gel electrophoresis apparatus for Ferguson plot analysis at the sub-microgram load level
Journal of Biochemical and Biophysical Methods, Vol.19(1), pp.105-120
1989
Abstract
A method for the simultaneous horizontal agarose gel electrophoresis on thin layer strips of different gel concentrations was developed for the purpose of generating Ferguson plots at the sub-microgram load level. Seven independent gel strips on a common GelBond support formed by filling channels created by a comb-shaped spacer (polycarbonate) in a vertical multistrip cassette. Electrophoresis on a horizontal Peltier-cooled surface employed commercial apparatus (E-C Apparatus Corp.) with a modified cover which is airtight and holds anodic and cathodic voltage measurement probes for each strip. The application of the apparatus to Ferguson plot analysis in a single experiment wa exemplified on the RNA-containing turnip crinkle virus (TCV) at a load of 50 ng/gel strip, using an optimized silver staining method (a modification of a procedure of FMC Corp. BioProducts) for detection. Within the range of 3.5 to 12.5 V/cm, the plot was found to be independent of field strength. Mobility is also independent of the concentration of detergent (CHAPS) up to 10 mM.
Details
- Title
- A thin-layer multistrip agarose gel electrophoresis apparatus for Ferguson plot analysis at the sub-microgram load level
- Authors/Creators
- L. Orbán (Author/Creator) - National Institute of Child HealthJ.V. Sullivan (Author/Creator) - National Institutes of HealthA. Chrambach (Author/Creator) - National Institute of Child Health
- Publication Details
- Journal of Biochemical and Biophysical Methods, Vol.19(1), pp.105-120
- Publisher
- Elsevier
- Identifiers
- 991005543707707891
- Copyright
- © 1989 Published by Elsevier B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Biophysics
- ESI research areas
- Biology & Biochemistry