Journal article
A three-year whole genome sequencing perspective of Enterococcus faecium sepsis in Australia
PLoS ONE, Vol.15(2), e0228781
2020
Abstract
Background
Over the last three decades, hospital adapted clonal complex (CC) 17 strains of Enterococcus faecium have acquired and exchanged antimicrobial resistance genes leading to the widespread resistance to clinically important antimicrobials globally. In Australia, a high prevalence of vancomycin resistance has been reported in E. faecium in the last decade.
Methods
In this study, we determined the phylogenetic relationship and genetic characteristics of E. faecium collected from hospitalized patients with blood stream infections throughout Australia from 2015 to 2017 using high throughput molecular techniques.
Results
Using single nucleotide polymorphism based phylogenetic inference, three distinct clusters of isolates were observed with additional sub-clustering. One cluster harboured mostly non-CC17 isolates while two clusters were dominant for the vanA and vanB operons.
Conclusion
The gradual increase in dominance of the respective van operon was observed in both the vanA and vanB dominant clusters suggesting a strain-van operon affinity. The high prevalence of the van operon within isolates of a particular sub-cluster was linked to an increased number of isolates and 30-day all-cause mortality. Different dominant sub-clusters were observed in each region of Australia. Findings from this study can be used to put future surveillance data into a broader perspective including the detection of novel E. faecium strains in Australia as well as the dissemination and evolution of each strain.
Details
- Title
- A three-year whole genome sequencing perspective of Enterococcus faecium sepsis in Australia
- Authors/Creators
- T. Lee (Author/Creator) - Murdoch UniversityS. Pang (Author/Creator) - Murdoch UniversityM. Stegger (Author/Creator) - Murdoch UniversityS. Sahibzada (Author/Creator) - Murdoch UniversityS. Abraham (Author/Creator) - Murdoch UniversityD. Daley (Author/Creator) - Fiona Stanley HospitalG. Coombs (Author/Creator) - Murdoch University
- Publication Details
- PLoS ONE, Vol.15(2), e0228781
- Publisher
- Public Library of Science
- Identifiers
- 991005540210407891
- Copyright
- © 2020 Lee et al.
- Murdoch Affiliation
- Antimicrobial Resistance and Infectious Disease Laboratory; School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.173 MRSA and VRE
- Web Of Science research areas
- Microbiology
- ESI research areas
- Biology & Biochemistry