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Journal article
A transcription factor gene CaMYB114 associated with black seed coat in chickpea
BMC plant biology, Vol.25(1), 1543
2025
Abstract
Background
Grain colour is an important quality trait affecting the market value and consumer preference. Chickpeas with black-coloured seed coat is known for their beneficial high antioxidant and fibber content, yet the underlying molecular basis remains poorly understood.
Results
Here, we examined the grain colour trait of a panel of 261 diverse desi chickpea (Cicer arietinum) accessions and specially characterized the development of the black seed coat. We showed that the black colouration emerged on embryo tips at 30 days after flowering (DAF) and expanded to whole grain at 35 DAF. Genome-wide association analyses revealed a single major genetic locus CaBlk3-1 on chromosome Ca3 controlling black seed coat. Candidate gene screening within 0.5 Mb upstream and downstream of CaBlk3-1 identified a single MYB-encoding gene CaMYB114 related to anthocyanin biosynthesis. Phylogeny analyses showed that CaMYB114 was clustered with Arabidopsis MYB90, MYB113, MYB114, consistent with their role in anthocyanin production. Subsequent qRT-PCR analyses suggested that CaMYB114 was abundantly transcribed in black genotypes but weakly in the brown genotypes at 35 DAF, closely linked with black colour development. Genetic variation analyses of CaMYB114 identified a 12-bp deletion containing a GAGA motif in the 5UTR region of black chickpea genotype. A gene-specific marker targeting this deletion was developed to validate its link with the black seed coat in a larger chickpea germplasm collection.
Conclusions
We identified a single major QTL and the underlying candidate gene CaMYB114 closely associated with the black seed coat trait in chickpea. Our study has greatly improved our understanding of the genetic basis of chickpea black seed and will unlock the potential for breeding new chickpeas with desired grain colour to meet various market requirements.
Details
- Title
- A transcription factor gene CaMYB114 associated with black seed coat in chickpea
- Authors/Creators
- Hao Luo - Murdoch UniversityGayathri Rathnayake - Western Crop Genetic Alliance, Murdoch University, State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, College of Science, Health, Engineering and Education, Murdoch UniversityLee-Anne McFawn - Department of Primary Industries and Regional DevelopmentJenifer Bussanich - Department of Primary Industries and Regional DevelopmentLan Lan - Murdoch UniversityZibei Lin - Jobs (Italy)Wangzhen Wu - Western Crop Genetic Alliance, Murdoch University, State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, College of Science, Health, Engineering and Education, Murdoch UniversityCong Tan - BGI Group (Japan)Sukhjiwan Kaur - Jobs (Italy)Darshan Sharma - Department of Primary Industries and Regional DevelopmentYong Jia - Western Crop Genetic Alliance, Murdoch University, State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, College of Science, Health, Engineering and Education, Murdoch UniversityChengdao Li - Western Crop Genetic Alliance, Murdoch University, State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, College of Science, Health, Engineering and Education, Murdoch University, Department of Primary Industries and Regional Development, College of Agriculture, Shandong Agricultural University
- Publication Details
- BMC plant biology, Vol.25(1), 1543
- Publisher
- BioMed Central Ltd unless otherwise stated. Part of Springer Nature.
- Grant note
- DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX; DAW2205-004RTX and UMU2303-003RTX / Grains Research and Development Corporation (https://doi.org/10.13039/501100000980)
- Identifiers
- 991005828072607891
- Copyright
- © The Author(s) 2025.
- Murdoch Affiliation
- Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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