ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling

Alice Goode; Sarah Rea; Melanie Sultana; Barry Shaw; Mark S Searle; Robert Layfield

doi:10.1016/j.mcn.2016.08.004

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ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling

Journal article

Open access

Peer reviewed

ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling

Alice Goode, Sarah Rea, Melanie Sultana, Barry Shaw, Mark S Searle and Robert Layfield

Molecular and cellular neurosciences, Vol.76, pp.52-58

2016

DOI: https://doi.org/10.1016/j.mcn.2016.08.004

PMCID: PMC5062946

PMID: 27554286

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Abstract

Amyotrophic Lateral Sclerosis - genetics

Binding Sites

Frontotemporal Lobar Degeneration - genetics

HEK293 Cells

Humans

Kelch-Like ECH-Associated Protein 1 - metabolism

Mutation, Missense

NF-E2-Related Factor 2 - metabolism

Protein Binding

Response Elements

Sequestosome-1 Protein - chemistry

Sequestosome-1 Protein - genetics

Sequestosome-1 Protein - metabolism

Signal Transduction

The transcription factor Nrf2 and its repressor protein Keap1 play key roles in the regulation of antioxidant stress responses and both Keap1-Nrf2 signalling and oxidative stress have been implicated in the pathogenesis of the ALS-FTLD spectrum of neurodegenerative disorders. The Keap1-binding partner and autophagy receptor SQSTM1/p62 has also recently been linked genetically to ALS-FTLD, with some missense mutations identified in patients mapping within or close to its Keap1-interacting region (KIR, residues 347-352). Here we report the effects on protein function of four different disease associated mutations of SQSTM1/p62 which affect the KIR region. Only mutations mapping precisely to the KIR (P348L and G351A) were associated with a loss of Keap1 binding in co-immunoprecipitations comparable to wild-type SQSTM1/p62. These selective effects on Keap1 recognition were entirely rational based on protein structural models. Consistent with impaired Keap1 binding, the P348L and G351A KIR mutants showed reduced ability to activate Nrf2 signalling compared to wild-type SQSTM1/p62 in antioxidant response element (ARE)-luciferase reporter assays. The results suggest that SQSTM1 mutations within the KIR of SQSTM1/p62 contribute to aetiology of some cases of ALS-FTLD through a mechanism involving aberrant expression or regulation of oxidative response genes.

Details

Title: ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling
Authors/Creators: Alice Goode - University of Nottingham
Sarah Rea - Harry Perkins Institute of Medical Research
Melanie Sultana - Sir Charles Gairdner Hospital
Barry Shaw - University of Nottingham
Mark S Searle - University of Nottingham
Robert Layfield - University of Nottingham
Publication Details: Molecular and cellular neurosciences, Vol.76, pp.52-58
Publisher: Elsevier Inc.
Grant note: LAYFIELD/APR13/821-791 / Motor Neurone Disease Association
Identifiers: 991005616477307891
Murdoch Affiliation: Centre for Molecular Medicine and Innovative Therapeutics
Language: English
Resource Type: Journal article

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Source: InCites

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.52 Neurodegenerative Diseases; 1.52.765 ALS Mechanisms
Web Of Science research areas: Neurosciences
ESI research areas: Neuroscience & Behavior