Journal article
Ability of different assay platforms to measure renal biomarker concentrations during ischaemia-reperfusion acute kidney injury in dogs
Research in Veterinary Science, Vol.135, pp.547-554
2021
Abstract
Several protein biomarkers have been shown to be useful for the early diagnosis of acute kidney injury (AKI) in animals and people. Multiplex assays for measurement of a panel of renal biomarkers in canine samples have recently become available. This study compared the use of two such assays, versus previously validated ELISAs, to measure five biomarkers in canine samples during ischaemia-reperfusion (IR) AKI. Blood and urine was collected from six male anaesthetised greyhounds that underwent 1-h of renal ischaemia (severe hypotension induced by acute haemorrhage) and 2-h of reperfusion (intravenous fluid resuscitation). Histology confirmed presence of acute tubular injury at 2 h of reperfusion. Concentrations of clusterin, cystatin C, kidney-injury molecule 1 (KIM-1), monocyte chemoattractant protein 1, and neutrophil gelatinase-associated lipocalin (NGAL) at baseline and following IR, measured by two different multiplex assays and previously-validated single analyte immunoassays, were compared. Only NGAL was significantly elevated following IR with all assays investigated. Whether concentrations of the other four biomarkers were significantly increased following IR depended on the assay used. Concentrations of cystatin C and KIM-1 measured with the multiplex assays were of a vast magnitude lower than those measured with the corresponding single analyte ELISAs. We conclude that further validation is required before these assays can reliably be used to measure AKI biomarkers in canine samples.
Details
- Title
- Ability of different assay platforms to measure renal biomarker concentrations during ischaemia-reperfusion acute kidney injury in dogs
- Authors/Creators
- J. Davis (Author/Creator) - Murdoch UniversityG. Rossi (Author/Creator) - Murdoch UniversityD.W. Miller (Author/Creator) - Murdoch UniversityR.E. Cianciolo (Author/Creator) - The Ohio State UniversityA.L. Raisis (Author/Creator) - Murdoch University
- Publication Details
- Research in Veterinary Science, Vol.135, pp.547-554
- Publisher
- Elsevier
- Identifiers
- 991005543737707891
- Copyright
- © 2020 Elsevier Ltd.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.55 Urology & Nephrology - General
- 1.55.830 Acute Kidney Injury
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science