Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments

T. Veltman; D. Jordan; C.A. McDevitt; J. Bell; B.P. Howden; M. Valcanis; M. O'Dea; S. Abraham; P. Scott; J.H. Kovac; R. Chia; B. Combs; K. Chousalkar; T. Wilson; D.J. Trott

doi:10.1016/j.ijfoodmicro.2021.109042

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Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments

Journal article

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Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments

T. Veltman, D. Jordan, C.A. McDevitt, J. Bell, B.P. Howden, M. Valcanis, M. O'Dea, S. Abraham, P. Scott, J.H. Kovac, …

International Journal of Food Microbiology, Vol.340, Art.109042

2021

DOI: https://doi.org/10.1016/j.ijfoodmicro.2021.109042

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Abstract

The development of antimicrobial resistance in foodborne pathogens is a growing public health concern. This study was undertaken to determine the antimicrobial susceptibility of Salmonella enterica subspecies enterica isolated from the Australian commercial egg layer industry. S. enterica subspecies enterica (n=307) isolated from Australian commercial layer flock environments (2015-2018) were obtained from reference, research and State Government laboratories from six Australian states. All Salmonella isolates were serotyped. Antimicrobial susceptibility testing (AST) for 16 antimicrobial agents was performed by broth microdilution. Antimicrobial resistance genes and sequence types (STs) were identified in significant isolates by whole genome sequencing (WGS). Three main serotypes were detected, S. Typhimurium (n=61, 19.9%), S. Senftenburg (n=45, 14.7%) and S. Agona (n=37, 12.1%). AST showed 293/307 (95.4%) isolates were susceptible to all tested antimicrobial agents and all isolates were susceptible to amoxicillin-clavulanate, azithromycin, ceftiofur, ceftriaxone, ciprofloxacin, colistin, florfenicol, gentamicin, kanamycin and trimethoprim-sulfamethoxazole. Low levels of non-susceptibility were observed to streptomycin (2.3%, n=7), sulfisoxazole (2.0%, n=6), chloramphenicol (1.3%, n=4) and tetracycline (1.0%, n=3). Very low levels of non-susceptibility were observed to ampicillin (2/307; 0.7%) and cefoxitin (2/307; 0.7%). Two isolates (S. Havana and S. Montevideo), exhibited multidrug-resistant phenotypes to streptomycin, sulfisoxazole and tetracycline and possessed corresponding antimicrobial resistance genes (aadA4, aac(6')-Iaa, sul1, tetB). One S. Typhimurium isolate was resistant to ampicillin and tetracycline, and possessed both tetA and blaTEM-1B. WGS also identified these isolates as belonging to ST4 (S. Montevideo), ST578 (S. Havana) and ST19 (S. Typhimurium). The absence of resistance to highest priority critically important antimicrobials as well as the extremely low level of AMR generally among Australian commercial egg layer Salmonella isolates likely reflect Australia’s conservative antimicrobial registration policy in food-producing animals and low rates of antimicrobial use within the industry.

Details

Title: Absence of high priority critically important antimicrobial resistance in Salmonella sp. isolated from Australian commercial egg layer environments
Authors/Creators: T. Veltman (Author/Creator) - The University of Adelaide
D. Jordan (Author/Creator) - New South Wales Department of Primary Industries
C.A. McDevitt (Author/Creator) - The University of Adelaide
J. Bell (Author/Creator) - The University of Adelaide
B.P. Howden (Author/Creator) - Peter Doherty Institute
M. Valcanis (Author/Creator) - Peter Doherty Institute
M. O'Dea (Author/Creator)
S. Abraham (Author/Creator) - Murdoch University
P. Scott (Author/Creator) - Scolexia Pty Ltd, Moonee Ponds, Australia.
J.H. Kovac (Author/Creator) - The University of Adelaide
R. Chia (Author/Creator) - Australian Eggs
B. Combs (Author/Creator) - Government of Western Australia Department of Health
K. Chousalkar (Author/Creator) - The University of Adelaide
T. Wilson (Author/Creator) - Scolexia Pty Ltd, Moonee Ponds, Australia.
D.J. Trott (Author/Creator) - The University of Adelaide
Publication Details: International Journal of Food Microbiology, Vol.340, Art.109042
Publisher: Elsevier
Identifiers: 991005542013007891
Copyright: © 2021 Elsevier B.V.
Murdoch Affiliation: College of Science, Health, Engineering and Education; Antimicrobial Resistance and Infectious Disease Laboratory
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.42 Bacteriology; 1.42.376 Salmonella and Campylobacter
Web Of Science research areas: Food Science & Technology; Microbiology
ESI research areas: Agricultural Sciences