Journal article
Absolute quantification of human milk caseins and the whey/casein ratio during the first year of lactation
Journal of Proteome Research, Vol.16(11), pp.4113-4121
2017
Abstract
Whey proteins and caseins in breast milk provide bioactivities and also have different amino acid composition. Accurate determination of these two major protein classes provides a better understanding of human milk composition and function, and further aids in developing improved infant formulas based on bovine whey proteins and caseins. In this study, we implemented a LC-MS/MS quantitative analysis based on iBAQ label-free quantitation, to estimate absolute concentrations of α-casein, β-casein, and κ-casein in human milk samples (n = 88) collected between day 1 and day 360 postpartum. Total protein concentration ranged from 2.03 to 17.52 with a mean of 9.37 ± 3.65 g/L. Casein subunits ranged from 0.04 to 1.68 g/L (α-), 0.04 to 4.42 g/L (β-), and 0.10 to 1.72 g/L (α-), with β-casein having the highest average concentration among the three subunits. Calculated whey/casein ratio ranged from 45:55 to 97:3. Linear regression analyses show significant decreases in total protein, β-casein, κ-casein, total casein, and a significant increase of whey/casein ratio during the course of lactation. Our study presents a novel and accurate quantitative analysis of human milk casein content, demonstrating a lower casein content than earlier believed, which has implications for improved infants formulas.
Details
- Title
- Absolute quantification of human milk caseins and the whey/casein ratio during the first year of lactation
- Authors/Creators
- Y. Liao (Author/Creator) - University of California, DavisD. Weber (Author/Creator) - University of California, DavisW. Xu (Author/Creator) - Murdoch UniversityB.P. Durbin-Johnson (Author/Creator) - University of California, DavisB.S. Phinney (Author/Creator) - University of California, DavisB. Lönnerdal (Author/Creator) - University of California, Davis
- Publication Details
- Journal of Proteome Research, Vol.16(11), pp.4113-4121
- Publisher
- American Chemical Society
- Identifiers
- 991005542059007891
- Copyright
- © 2017 American Chemical Society.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.784 Dairy Product Chemistry
- Web Of Science research areas
- Biochemical Research Methods
- ESI research areas
- Biology & Biochemistry