Journal article
Accelerated separation of random complex DNA patterns in gels: Comparing the performance of discontinuous and continuous buffers
Electrophoresis, Vol.20(7), pp.1462-1468
1999
Abstract
Complex DNA fragment patterns produced by random amplified polymorphic DNA (RAPD) assays were separated in agarose as well as polyacrylamide gels using a continuous buffer and two discontinuous buffer systems, with and without urea, respectively. The effect of very high field strength / current levels on the duration of separation, the relative mobilities of the selected bands, and the appearance of the pattern was tested. The use of discontinuous buffer systems resulted in sharper bands and better resolution at 30 min duration of separation for DNA fragments in the size range of 200—2000 base pairs. These observations could help researchers in the rapid separation of band patterns produced by polymerase chain reaction (PCR)-based methods utilized in small- to mid-scale genome searches.
Details
- Title
- Accelerated separation of random complex DNA patterns in gels: Comparing the performance of discontinuous and continuous buffers
- Authors/Creators
- A. Zsolnai (Author/Creator)L. Orbán (Author/Creator)
- Publication Details
- Electrophoresis, Vol.20(7), pp.1462-1468
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005542548707891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- International collaboration
- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry