Actinomycins from Soil-Inhabiting Streptomyces as Sources of Antibacterial Pigments for Silk Dyeing

Tananya Nuanjohn; Nungruthai Suphrom; Nareeluk Nakaew; Wasu Pathom-Aree; Nattha Pensupa; Apiradee Siangsuepchart; Bernard Dell; Juangjun Jumpathong

doi:10.3390/molecules28165949

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Actinomycins from Soil-Inhabiting Streptomyces as Sources of Antibacterial Pigments for Silk Dyeing

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Actinomycins from Soil-Inhabiting Streptomyces as Sources of Antibacterial Pigments for Silk Dyeing

Tananya Nuanjohn, Nungruthai Suphrom, Nareeluk Nakaew, Wasu Pathom-Aree, Nattha Pensupa, Apiradee Siangsuepchart, Bernard Dell and Juangjun Jumpathong

Molecules (Basel, Switzerland), Vol.28(16), 5949

2023

DOI: https://doi.org/10.3390/molecules28165949

PMID: 37630201

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Abstract

Actinobacteria produce a broad spectrum of bioactive substances that are used in the pharmaceutical, agricultural, and biotechnology industries. This study investigates the production of bioactive substances in Streptomyces, isolated from soil under five tropical plants, focusing on their potential as natural antibacterial dyes for silk fabrics. Out of 194 isolates, 44 produced pigments on broken rice as a solid substrate culture. Eight antibacterial pigmented isolates from under Magnolia baillonii (TBRC 15924, TBRC 15927, TBRC 15931), Magnolia rajaniana (TBRC 15925, TBRC 15926, TBRC 15928, TBRC 15930), and Cinnamomum parthenoxylon (TBRC 15929) were studied in more detail. TBRC 15927 was the only isolate where all the crude extracts inhibited the growth of the test organisms, Staphylococcus epidermidis TISTR 518 and S. aureus DMST 4745. The bioactive compounds present in TBRC 15927 were identified through LC-MS/MS analysis as belonging to the actinomycin group, actinomycin D (or X1), X2, and X0β. Also, the ethyl acetate crude extract exhibited non-toxicity at an IC50 value of 0.029 ± 0.008 µg/mL on the mouse fibroblast L-929 assay. From the 16S rRNA gene sequence analysis, TBRC 15927 had 100% identity with Streptomyces gramineus JR-43T. Raw silk dyed with the positive antimicrobial TBRC 15927 extract (8.35 mg/mL) had significant (>99.99%) antibacterial properties. Streptomyces gramineus TBRC 15927 is the first actinomycin-producing strain reported to grow on broken rice and shows promise for antibacterial silk dyeing.

Details

Title: Actinomycins from Soil-Inhabiting Streptomyces as Sources of Antibacterial Pigments for Silk Dyeing
Authors/Creators: Tananya Nuanjohn - Naresuan University
Nungruthai Suphrom - Naresuan University
Nareeluk Nakaew - Naresuan University
Wasu Pathom-Aree - Chiang Mai University
Nattha Pensupa - Naresuan University
Apiradee Siangsuepchart - Maejo University
Bernard Dell - Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand Department of Chemistry, Faculty of Science, Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand Research Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand Department of Agro-Industrial Biotechnology, Maejo University Phrae Campus, Phrae 54140, Thailand School of Agricultural Sciences, Murdoch University, Perth 6150, Australia Center of Excellence in Research for Agricultural Biotechnology, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
Juangjun Jumpathong - Naresuan University
Publication Details: Molecules (Basel, Switzerland), Vol.28(16), 5949
Publisher: MDPI
Grant note: R2566C051 / Global and Frontier Research University Fund, Naresuan University
Identifiers: 991005601369207891
Copyright: © 2023 by the authors.
Murdoch Affiliation: School of Agricultural Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.198 Mycotoxins; 3.198.904 Streptomyces
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
ESI research areas: Chemistry