Journal article
Adipose tissue is enriched for activated and Late-differentiated CD8+ T cells, and shows distinct CD8+ receptor usage, compared to blood in HIV-infected persons
JAIDS Journal of Acquired Immune Deficiency Syndromes, Vol.77(2), pp.e14-e21
2017
Abstract
BACKGROUND:
Adverse viral and medication effects on adipose tissue contribute to the development of metabolic disease in HIV-infected persons, but T cells also have a central role modulating local inflammation and adipocyte function. We sought to characterize potentially proinflammatory T-cell populations in adipose tissue among persons on long-term antiretroviral therapy and assess whether adipose tissue CD8 T cells represent an expanded, oligoclonal population.
METHODS:
We recruited 10 HIV-infected, non-diabetic, overweight or obese adults on efavirenz, tenofovir, and emtricitabine for >4 years with consistent viral suppression. We collected fasting blood and subcutaneous abdominal adipose tissue to measure the percentage of CD4 and CD8 T cells expressing activation, exhaustion, late differentiation/senescence, and memory surface markers. We performed T-cell receptor (TCR) sequencing on sorted CD8 cells. We compared the proportion of each T-cell subset and the TCR repertoire diversity, in blood versus adipose tissue.
RESULTS:
Adipose tissue had a higher percentage of CD3CD8 T cells compared with blood (61.0% vs. 51.7%, P < 0.01) and was enriched for both activated CD8HLA-DR T cells (5.5% vs. 0.9%, P < 0.01) and late-differentiated CD8CD57 T cells (37.4% vs. 22.7%, P < 0.01). Adipose tissue CD8 T cells displayed distinct TCRβ V and J gene usage, and the Shannon Entropy index, a measure of overall TCRβ repertoire diversity, was lower compared with blood (4.39 vs. 4.46; P = 0.05).
CONCLUSIONS:
Adipose tissue is enriched for activated and late-differentiated CD8 T cells with distinct TCR usage. These cells may contribute to tissue inflammation and impaired adipocyte fitness in HIV-infected persons.
Details
- Title
- Adipose tissue is enriched for activated and Late-differentiated CD8+ T cells, and shows distinct CD8+ receptor usage, compared to blood in HIV-infected persons
- Authors/Creators
- J.R. Koethe (Author/Creator)W. McDonnell (Author/Creator)A. Kennedy (Author/Creator)C.O. Abana (Author/Creator)M. Pilkinton (Author/Creator)I. Setliff (Author/Creator)I. Georgiev (Author/Creator)L. Barnett (Author/Creator)C.C. Hager (Author/Creator)R. Smith (Author/Creator)S.A. Kalams (Author/Creator)A. Hasty (Author/Creator)S. Mallal (Author/Creator)
- Publication Details
- JAIDS Journal of Acquired Immune Deficiency Syndromes, Vol.77(2), pp.e14-e21
- Publisher
- Lippincott Williams & Wilkins
- Identifiers
- 991005543869107891
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.66 HIV
- 1.66.1372 HIV Comorbidities
- Web Of Science research areas
- Immunology
- Infectious Diseases
- ESI research areas
- Immunology