Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide

M.A. Islam; M.J. Uddin; E. Tholen; D. Tesfaye; C. Looft; K. Schellander; M.U. Çınar

doi:10.1016/j.tvjl.2013.07.026

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Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide

Journal article

Peer reviewed

Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide

M.A. Islam, M.J. Uddin, E. Tholen, D. Tesfaye, C. Looft, K. Schellander and M.U. Çınar

The Veterinary Journal, Vol.198(1), pp.245-251

2013

DOI: https://doi.org/10.1016/j.tvjl.2013.07.026

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Abstract

The aim of the present study was to investigate the age-related production variation of T helper (Th)-type cytokines (IL-2, IL-4, IFN-γ and IL-10), granulocyte macrophage-colony stimulating factor (GM-CSF) and nitric oxide (NO) by lipopolysaccharide (LPS)-stimulated porcine alveolar macrophages (AMs) in a time-dependent manner. For this purpose, AMs were isolated from 5-days (newborn), 40-days (post-weaned) and 120-days (young) old pigs. Cells were incubated for 24 h in the absence or presence of increasing concentrations of LPS (0.0, 0.01, 1.0, 5.0 and 10.0 μg/mL). IL-10, IFN-γ and GM-CSF mRNA expression was upregulated in a dose-dependent manner for all age groups (P < 0.05). Age-related differences included a significantly increased IL-10 mRNA and protein production in newborn piglets compared to post-weaned and young pigs. IL-10 production pattern was similar with a higher peak between 12 and 36 h post-induction in all age groups. In contrast, IFN-γ mRNA and protein level was significantly elevated in young pigs 12 h and 24 h post-induction, respectively, while the time course production of IFN-γ was mostly consistent in newborn and post-weaned piglets. GM-CSF mRNA expression was significantly lower in newborn piglets than in post-weaned and young pigs. The kinetic of GM-CSF expression peaked at 12 h in young and post-weaned pigs and at 24 h in newborn piglets. IL-4 mRNA levels were very low and no apparent change of IL-2 expression was observed following LPS stimulation in all age groups. Only very low levels of NO were detected in the cell supernatants of young pigs. Collectively, these studies suggest age-related differences in time-dependent production of IL-10, IFN-γ and GM-CSF by porcine AMs with potential immunoregulatory consequences to be explored further.

Details

Title: Age-associated differential production of IFN-γ, IL-10 and GM-CSF by porcine alveolar macrophages in response to lipopolysaccharide
Authors/Creators: M.A. Islam (Author/Creator)
M.J. Uddin (Author/Creator)
E. Tholen (Author/Creator)
D. Tesfaye (Author/Creator)
C. Looft (Author/Creator)
K. Schellander (Author/Creator)
M.U. Çınar (Author/Creator)
Publication Details: The Veterinary Journal, Vol.198(1), pp.245-251
Publisher: Balliere Tindall
Identifiers: 991005543734307891
Copyright: © 2013 Elsevier Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.104 Virology - General; 1.104.1933 Porcine Viral Challenges
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science