Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles

C.Y. Yu; N.M. Boyd; S.J. Cringle; E.N. Su; V.A. Alder; D.Y. Yu

doi:10.1016/S0003-9969(01)00107-8

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Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles

Journal article

Peer reviewed

Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles

C.Y. Yu, N.M. Boyd, S.J. Cringle, E.N. Su, V.A. Alder and D.Y. Yu

Archives of Oral Biology, Vol.47(2), pp.99-107

2002

DOI: https://doi.org/10.1016/S0003-9969(01)00107-8

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Abstract

A novel isolated perfused pulpal arteriole preparation and microperfusion system was used to evaluate the direct vasoactive responses of pulpal arterioles to selected agonists. Short lengths of porcine pulpal arterioles (101.7 ± 2.2 μm o.d., n = 105) were dissected out and placed in an environment-controlled bath on the stage of an inverted microscope. Both ends of the vessel were cannulated and perfused at a controlled rate through the lumen. The diameter of the vessel was measured online. Following equilibration, the vessel was challenged with various agonists: adrenaline (epinephrine), noradrenaline (norepinephrine), phenylephrine, dopamine, isoproterenol, 5-hydroxytryptamine, histamine and adenosine. The endothelium-dependent vasodilator acetylcholine was used to evaluate endothelial cell function. Adrenaline, noradrenaline, phenylephrine, 5-hydroxytryptamine and dopamine caused dose-dependent contractions (adrenaline = noradrenaline > phenylephrine > dopamine > 5-hydroxytryptamine). Isoproterenol and histamine provoked a dose-dependent dilation. Adenosine produced pronounced vasodilatation in vessels precontracted with 10-8 M endothelin-1. Functional adrenergic, histamine, 5-hydroxytryptamine and adenosine receptors are, therefore, present in porcine pulpal arterioles. The isolated perfused pulpal arteriole preparation may prove valuable in understanding local control mechanisms of pulpal microcirculation.

Details

Title: Agonist-induced vasoactive responses in isolated perfused porcine dental pulpal arterioles
Authors/Creators: C.Y. Yu (Author/Creator) - The University of Western Australia
N.M. Boyd (Author/Creator) - The University of Western Australia
S.J. Cringle (Author/Creator) - The University of Western Australia
E.N. Su (Author/Creator) - The University of Western Australia
V.A. Alder (Author/Creator) - Murdoch University
D.Y. Yu (Author/Creator) - Murdoch University
Publication Details: Archives of Oral Biology, Vol.47(2), pp.99-107
Publisher: Elsevier
Identifiers: 991005542226707891
Copyright: © 2002 Elsevier Science Ltd.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.195 Neuroendocrine & Intestinal Disorders; 1.195.578 Neuropeptide Roles
Web Of Science research areas: Dentistry, Oral Surgery & Medicine
ESI research areas: Clinical Medicine