Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6

K. Dong; P. Ge; C. Ma; K. Wang; X. Yan; L. Gao; X. Li; J. Liu; W. Ma; Y. Yan

doi:10.1016/j.jcs.2012.08.016

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Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6

Journal article

Open access

Peer reviewed

Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6

K. Dong, P. Ge, C. Ma, K. Wang, X. Yan, L. Gao, X. Li, J. Liu, W. Ma and Y. Yan

Journal of Cereal Science, Vol.56(3), pp.615-622

2012

DOI: https://doi.org/10.1016/j.jcs.2012.08.016

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Abstract

Proteome Dynamics of albumins and globulins during grain development of elite Chinese wheat cultivar Xiaoyan 6 were studied. A total of 210 differentially expressed protein spots were recognized by two-dimensional differential gel electrophoresis (2D-DIGE) and 146 of these that represented 89 unique proteins were identified by MALDI-TOF mass spectrometry. Four protein expression patterns were observed across five grain developmental stages. Among the identified proteins, more than 80% were enzymes in eight functional categories, including carbohydrate metabolism (27%), protein metabolism (27%), stress/defense/detoxification (11%), cell metabolism (6%), transcription/translation (4%), nitrogen metabolism (4%), photosynthesis (4%), and signal transduction (1%). Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 18 representative genes demonstrated that about two thirds of the genes displayed different expression profiles between the transcriptional and translational stages.

Details

Title: Albumin and globulin dynamics during grain development of elite Chinese wheat cultivar Xiaoyan 6
Authors/Creators: K. Dong (Author/Creator)
P. Ge (Author/Creator)
C. Ma (Author/Creator)
K. Wang (Author/Creator)
X. Yan (Author/Creator)
L. Gao (Author/Creator)
X. Li (Author/Creator)
J. Liu (Author/Creator)
W. Ma (Author/Creator)
Y. Yan (Author/Creator)
Publication Details: Journal of Cereal Science, Vol.56(3), pp.615-622
Publisher: Academic Press
Identifiers: 991005542502307891
Copyright: © 2012 Elsevier Ltd.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Food Science & Technology
ESI research areas: Agricultural Sciences