Journal article
Allele-specific HLA-B*15 typing by PCR-SSP and its application to four distinct ethnic populations
Tissue Antigens, Vol.51(3), pp.293-300
2008
Abstract
We present a set of primer mixes for the allele-specific typing of the HLA-B*15 group by PCR-SSP. The set comprises 46 primer mixes which are designed to unequivocally resolve all but two of the 666 possible combinations of the B*15 alleles, B*1501–37 (B*1536 sequence unavailable). A core subset of 34 of the 46 mixes can be used alone to give a high resolution B*15 typing set. This allows for the identification of each B*15 allele when present as the only B*15 allele and the majority of the possible B*15 homozygotic combinations. The method was validated using reference DNA samples and the B*15 allele frequency in 4 distinct ethnic populations was investigated. The results show that these populations contain predominantly mutually exclusive sets of B*15 alleles.
Details
- Title
- Allele-specific HLA-B*15 typing by PCR-SSP and its application to four distinct ethnic populations
- Authors/Creators
- M.C.N.M. Barnardo (Author/Creator)K.I. Welsh (Author/Creator)C. Vilches (Author/Creator)K. Maitland (Author/Creator)M. Bunce (Author/Creator)
- Publication Details
- Tissue Antigens, Vol.51(3), pp.293-300
- Publisher
- Blackwell Publishing
- Identifiers
- 991005542165807891
- Copyright
- 1998 Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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