Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Y. Peng; Z. Yu; S. Islam; Y. Zhang; X. Wang; Z. Lei; K. Yu; D. Sun; W. Ma

doi:10.1270/jsbbs.16050

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Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Journal article

Peer reviewed

Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS

Y. Peng, Z. Yu, S. Islam, Y. Zhang, X. Wang, Z. Lei, K. Yu, D. Sun and W. Ma

Breeding Science, Vol.66(4), pp.646-652

2016

DOI: https://doi.org/10.1270/jsbbs.16050

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Abstract

Low molecular weight glutenin subunits are important components of wheat storage proteins, which play an important role in determining end-use quality of common wheat. A newly established matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) procedure was used to analyze 478 landraces of bread wheat collected from the Yangtze-River region in China. Results indicated that 17 alleles at three loci: Glu-A3, Glu-B3 and Glu-D3 were identified, resulting in 87 different allele combinations. Of the 17 alleles detected at all the Glu-3 loci, five belonged to Glu-A3, seven to Glu-B3 and five to Glu-D3 locus. MALDI-TOF-MS indicated Glu-A3a/c was present in 72.8%, Glu-A3b in 8.4%, Glu-A3d in 8.4%, Glu-A3f in 5.2% and Glu-A3e in 3.6% lines. Seven types of alleles were identified at the Glu-B3 locus: Glu-B3d/i (25.5%), Glu-B3b (21.3%), Glu-B3c (16.9%), Glu-B3h (13.8%), Glu-B3f (8.4%), Glu-B3a (8.2%), and Glu-B3g (5.2%). Five types of Glu-D3 alleles were detected: Glu-D3a (58.4%), Glu-D3c (22.6%), Glu-D3d (15.5%), Glu-D3b (3.3%) and Glu-D3f (0.2%). Four new alleles that showed abnormal MALDI-TOF spectrum patterns were identified at the Glu-A3 and Glu-B3 loci. A detailed study is needed to further characterize these alleles and their potential usage for wheat improvement.

Details

Title: Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS
Authors/Creators: Y. Peng (Author/Creator) - Huazhong Agricultural University
Z. Yu (Author/Creator) - Australian Export Grains Innovation Centre
S. Islam (Author/Creator) - Technology Centre Prague
Y. Zhang (Author/Creator) - Technology Centre Prague
X. Wang (Author/Creator) - Technology Centre Prague
Z. Lei (Author/Creator) - Henan Academy of Agricultural Sciences
K. Yu (Author/Creator) - Huazhong Agricultural University
D. Sun (Author/Creator) - Huazhong Agricultural University
W. Ma (Author/Creator) - Technology Centre Prague
Publication Details: Breeding Science, Vol.66(4), pp.646-652
Publisher: Japanese Society of Breeding
Identifiers: 991005542016407891
Copyright: © 2016 by JAPANESE SOCIETY OF BREEDING
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Agronomy; Plant Sciences
ESI research areas: Plant & Animal Science