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Allogeneic hematopoietic stem cell transplantation recipients have defects of both switched and IgM memory B cells
Journal article   Peer reviewed

Allogeneic hematopoietic stem cell transplantation recipients have defects of both switched and IgM memory B cells

L.J. D'Orsogna, M.P. Wright, R.G. Krueger, E.J. McKinnon, S.I. Buffery, C.S. Witt, N. Staples, R. Loh, P.K. Cannell, F.T. Christiansen, …
Biology of Blood and Marrow Transplantation, Vol.15(7), pp.795-803
2009
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Abstract

Allogeneic hematopoietic stem cell transplant (HSCT) recipients were assessed to elucidate memory B cell defects underlying their increased susceptibility to infections, particularly by encapsulated bacteria. Circulating IgM memory B cells (CD19+, CD27+, IgM+) and switched memory B cells (CD19+, CD27+, IgM-) were enumerated in allogeneic HSCT recipients (n = 37) and healthy controls (n = 35). T lymphocyte subpopulations and serum levels of immunoglobulins, including IgG subclasses, and antibodies to pneumococcal polysaccharides were also assayed. Allogeneic HSCT recipients were deficient in both switched memory and IgM memory B cells compared to healthy controls (both P < .0001), irrespective of time post-HSCT. Switched memory B cell deficiency correlated with CD4+ T cell deficiency, and both correlated with serum levels of IgG1 (P < .0001), possibly reflecting impaired B cell isotype switching in germinal centres. "Steady-state" serum levels of antibodies to pneumococcal polysaccharides did not correlate with circulating memory B cells. Graft-versus-host disease (GVHD) was associated with lower IgM memory B cell counts and lower serum levels of IgG2, IgG4, IgA, and pneumococcal antibodies. The increased susceptibility of allogeneic HSCT patients to infection may reflect a combination of memory B cell defects, which are most common in patients with a history of GVHD.

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Collaboration types
Domestic collaboration
Citation topics
1 Clinical & Life Sciences
1.103 Blood Disorders
1.103.380 GvHD
Web Of Science research areas
Hematology
Immunology
Transplantation
ESI research areas
Immunology
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