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Alterations in phage-typing patterns in vancomycin-intermediate Staphylococcus aureus
Journal article   Peer reviewed

Alterations in phage-typing patterns in vancomycin-intermediate Staphylococcus aureus

J.E. Gustafson, F.G. O'Brien, G.W. Coombs, M.J. Malkowski, W.B. Grubb, R.F. Pfeltz and B.J. Wilkinson
Journal of Medical Microbiology, Vol.52(8), pp.711-714
2003
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Abstract

The ability of phage-typing and Smal chromosomal RFLPs to conclude appropriate strain relatedness between a collection of 12 well-characterized in vitro-selected vancomycin-intermediate Staphylococcus aureus (VISA) strains and their seven vancomycin-susceptible parent strains is reported. Generally, no Smal RFLP alterations were observed in VISA strains when they were compared with their respective parent strains, and clonal relationships between isogenic strains were clearly evident. Unlike the Smal RFLP results, parent strains and VISA derivatives generally did not share similar phage-typing profiles. Depending on the phage set investigated, some VISA strains even became untypable by this method. Loss of phage infectivity is probably due to cell wall (phage receptor) alterations that are expressed by the VISA strains investigated. Collectively, these findings indicate that inappropriate relationships between VISA and vancomycin-susceptible parents might be drawn if only phage-typing and antibiotic susceptibility are utilized to determine epidemiological relationships.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
1 Clinical & Life Sciences
1.23 Antibiotics & Antimicrobials
1.23.173 MRSA and VRE
Web Of Science research areas
Microbiology
ESI research areas
Microbiology
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