Journal article
Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant
Theoretical and Applied Genetics, Vol.133(1), pp.163-177
2020
Abstract
Tillering and plant height are important traits determining plant architecture and grain production in cereal crops. This study identified an excess-tillering semi-dwarf mutant (htd) from an EMS-treated barley population. Genetic analysis of the F1, F2, and F2:3 populations showed that a single recessive gene controlled the excess-tillering semi-dwarf in htd. Using BSR-Seq and gene mapping, the Hvhtd gene was delimited within a 1.8 Mb interval on chromosome 2HL. Alignment of the RNA-Seq data with the functional genes in the interval identified a gene HORVU2Hr1G098820 with alternative splicing between exon2 and exon3 in the mutant, due to a G to A single-nucleotide substitution at the exon and intron junction. An independent mutant with a similar phenotype confirmed the result, with alternative splicing between exon3 and exon4. In both cases, the alternative splicing resulted in a non-functional protein. And the gene HORVU2Hr1G098820 encodes a trypsin family protein and may be involved in the IAA signaling pathway and differs from the mechanism of Green Revolution genes in the gibberellic acid metabolic pathway.
Details
- Title
- Alternative splicing of a barley gene results in an excess-tillering and semi-dwarf mutant
- Authors/Creators
- W. Hua (Author/Creator)C. Tan (Author/Creator)J. Xie (Author/Creator)J. Zhu (Author/Creator)Y. Shang (Author/Creator)J. Yang (Author/Creator)X-Q Zhang (Author/Creator)X. Wu (Author/Creator)J. Wang (Author/Creator)C. Li (Author/Creator)
- Publication Details
- Theoretical and Applied Genetics, Vol.133(1), pp.163-177
- Publisher
- Springer Verlag
- Identifiers
- 991005545260307891
- Copyright
- © 2019 Springer-Verlag GmbH Germany, part of Springer Nature
- Murdoch Affiliation
- Western Barley Genetics Alliance
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Agricultural Sciences