Journal article
Aluminium toxicity in rye (Secale cereale): Root growth and dynamics of cytoplasmic Ca2+ in intact root tips
Annals of Botany, Vol.89(2), pp.241-244
2002
Abstract
Aluminium (Al.) toxicity in rye (Secale cereale L.), an Al-resistant crop, was examined by measuring root elongation and cytoplasmic free activity of calcium ([Ca2+]cyt) in intact root apical cells. Measurement of [Ca2+]cyt was achieved by loading a Ca2+-sensitive fluorescent probe, Fluo-3/AM ester, into root apical cells followed by detection of intracellular fluorescence using a confocal laser scanning microscope. After 20 min of exposure to 50 μM Al (pH 4.2) a slight increase in [Ca2+]cyt of root apical cells was observed, while the response of [Ca2+]cyt to 100 μM Al (pH 4.2) was faster and larger ([Ca2+]cyt increased by 46% in 10 min). Increases in [Ca2+]cyt were correlated with inhibition of root growth, generally measurable after 2 h. Addition of 400 μM malic acid (pH 4.2) largely ameliorated the effect of 100 μM Al on [Ca2+]cyt in root apical cells and protected root growth from Al toxicity. These results suggest that an increase in [Ca2+]cyt in root apical cells in rye is an early effect of Al toxicity and is followed by the secondary effect on root elongation.
Details
- Title
- Aluminium toxicity in rye (Secale cereale): Root growth and dynamics of cytoplasmic Ca2+ in intact root tips
- Authors/Creators
- Q. Ma (Author/Creator) - The University of Western Australia
- Publication Details
- Annals of Botany, Vol.89(2), pp.241-244
- Publisher
- Oxford University Press
- Identifiers
- 991005543671007891
- Copyright
- © 2002 Annals of Botany Company
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
43 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.473 Soil Phosphorus Dynamics
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science