Journal article
Alzheimer’s disease cerebrospinal fluid biomarkers are not influenced by gravity drip or aspiration extraction methodology
Alzheimer's Research & Therapy, Vol.7(1), Art. 71
2015
Abstract
Introduction: Cerebrospinal fluid (CSF) biomarkers, although of established utility in the diagnostic evaluation of Alzheimer's disease (AD), are known to be sensitive to variation based on pre-analytical sample processing. We assessed whether gravity droplet collection versus syringe aspiration was another factor influencing CSF biomarker analyte concentrations and reproducibility.
Methods: Standardized lumbar puncture using small calibre atraumatic spinal needles and CSF collection using gravity fed collection followed by syringe aspirated extraction was performed in a sample of elderly individuals participating in a large long-term observational research trial. Analyte assay concentrations were compared.
Results: For the 44 total paired samples of gravity collection and aspiration, reproducibility was high for biomarker CSF analyte assay concentrations (concordance correlation [95%CI]: beta-amyloid1-42 (Aβ42) 0.83 [0.71 - 0.90]), t-tau 0.99 [0.98 - 0.99], and phosphorylated tau (p-tau) 0.82 [95 % CI 0.71 - 0.89]) and Bonferroni corrected paired sample t-tests showed no significant differences (group means (SD): Aβ42 366.5 (86.8) vs 354.3 (82.6), p = 0.10; t-tau 83.9 (46.6) vs 84.7 (47.4) p = 0.49; p-tau 43.5 (22.8) vs 40.0 (17.7), p = 0.05). The mean duration of collection was 10.9 minutes for gravity collection and <1 minute for aspiration.
Conclusions: Our results demonstrate that aspiration of CSF is comparable to gravity droplet collection for AD biomarker analyses but could considerably accelerate throughput and improve the procedural tolerability for assessment of CSF biomarkers.
Details
- Title
- Alzheimer’s disease cerebrospinal fluid biomarkers are not influenced by gravity drip or aspiration extraction methodology
- Authors/Creators
- A. Rembach (Author/Creator) - Florey Institute of Neuroscience and Mental HealthL.A. Evered (Author/Creator) - St Vincent's HealthQ-X Li (Author/Creator) - Florey Institute of Neuroscience and Mental HealthT. Nash (Author/Creator) - Florey Institute of Neuroscience and Mental HealthL. Vidaurre (Author/Creator) - Florey Institute of Neuroscience and Mental HealthC.J. Fowler (Author/Creator) - Florey Institute of Neuroscience and Mental HealthK.K. Pertile (Author/Creator) - Florey Institute of Neuroscience and Mental HealthR.L. Rumble (Author/Creator) - Florey Institute of Neuroscience and Mental HealthB.O. Trounson (Author/Creator) - Florey Institute of Neuroscience and Mental HealthS. Maher (Author/Creator) - St Vincent's HealthF. Mooney (Author/Creator) - St Vincent's HealthM. Farrow (Author/Creator) - Dementia AustraliaK. Taddei (Author/Creator) - Edith Cowan UniversityS. Rainey-Smith (Author/Creator)S.M. Laws (Author/Creator) - Edith Cowan UniversityS.L. Macaulay (Author/Creator) - Preventative HealthW. Wilson (Author/Creator) - Australian e-Health Research CentreD.G. Darby (Author/Creator) - Florey Institute of Neuroscience and Mental HealthR.N. Martins (Author/Creator) - Edith Cowan UniversityD. Ames (Author/Creator) - National Ageing Research InstituteS. Collins (Author/Creator) - The University of MelbourneB. Silbert (Author/Creator) - St Vincent's HealthC.L. Masters (Author/Creator) - Florey Institute of Neuroscience and Mental HealthJ.D. Doecke (Author/Creator) - Preventative Health
- Publication Details
- Alzheimer's Research & Therapy, Vol.7(1), Art. 71
- Publisher
- Biomed Central
- Identifiers
- 991005542309507891
- Copyright
- © 2015 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- 1.52 Neurodegenerative Diseases
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