An In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum

Y-H Chooi; C. Krill; R.A. Barrow; S. Chen; R. Trengove; R.P. Oliver; P.S. Solomon

doi:10.1128/AEM.02745-14

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An In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum

Journal article

Peer reviewed

An In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum

Y-H Chooi, C. Krill, R.A. Barrow, S. Chen, R. Trengove, R.P. Oliver and P.S. Solomon

Applied and Environmental Microbiology, Vol.81(1), pp.177-186

2015

DOI: https://doi.org/10.1128/AEM.02745-14

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Abstract

Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ΔSN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 μg/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases.

Details

Title: An In planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum
Authors/Creators: Y-H Chooi (Author/Creator) - Australian National University
C. Krill (Author/Creator) - Murdoch University
R.A. Barrow (Author/Creator) - Australian National University
S. Chen (Author/Creator) - Australian National University
R. Trengove (Author/Creator) - Murdoch University
R.P. Oliver (Author/Creator) - Curtin University
P.S. Solomon (Author/Creator) - Australian National University
Publication Details: Applied and Environmental Microbiology, Vol.81(1), pp.177-186
Publisher: American Society for Microbiology
Identifiers: 991005540081707891
Copyright: © 2015, American Society for Microbiology.
Murdoch Affiliation: Separation Science and Metabolomics Laboratory
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.198 Mycotoxins; 3.198.926 Endophytic Fungi
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Biology & Biochemistry