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Journal article
An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia
Ecology and evolution, Vol.15(4), e71298
2025
PMID: 40297321
Appears in Open Access via Read & Publish Agreements
Abstract
We describe and characterize a new Isospora species from the black-faced cuckoo-shrike (Coracina novaehollandiae) in Western Australia, using both morphological and molecular approaches. Microscopic analysis of a fecal sample revealed 20 ellipsoidal oocysts, which are subspheroidal to ovoid, measuring 40–43 × 39–41 μm (mean 41.4 × 39.6 μm) with a length/width (L/W) ratio of 1.0–1.1 (mean 1.04). The oocyst wall is bi-layered (~1.5 μm thick), with a smooth outer layer constituting approximately two thirds of the total thickness. A micropyle is present, characterized by a slight invagination of the inner layer (~6.0 μm wide), but no micropyle cap is observed. Both the oocyst residuum and polar granule are absent. Sporocysts (n = 20) are ellipsoidal, measuring 23–24 × 13–14 μm (mean 23.3 × 13.4 μm) with an L/W ratio of 1.7–1.8 (mean 1.74). A flattened to knob-like Stieda body (~1.5 × 3.0 μm) is present, while sub-Stieda and para-Stieda bodies are absent. The sporocyst residuum is composed of granules clustered by a membrane, forming an irregular shape (~12.0 × 8.0 μm). Sporozoites exhibit anterior and posterior refractile bodies and a nucleus. Molecular analyses of the 18S rRNA, 28S rRNA, and COI gene loci demonstrated that this species forms a distinct clade with Isospora serinuse in phylogenetic trees based on all three loci. It shares genetic similarities with I. serinuse of 98.8%, 93.7%, and 98.9% for the 18S rRNA, 28S rRNA, and COI loci, respectively. Phylogenetic analysis confirms that this new species is closely related to I. serinuse. Based on these findings, we propose this isolate as a new species, Isospora coracinae n. sp. This is the first coccidian species identified from the Campephagidae family in Australia.
Details
- Title
- An Isospora Species (Apicomplexa: Eimeriidae) Identified From a Black‐Faced Cuckoo‐Shrike (Coracina novaehollandiae) (Gmelin, 1789) (Passeriformes: Campephagidae) in Western Australia
- Authors/Creators
- Yinhua Chen - Maotai InstituteBelinda Brice - Kanyana Wildlife Rehabilitation CentreBruno P. Berto - Universidade Federal Rural do Rio de JaneiroQiong Li - Maotai InstituteRongchang Yang - Murdoch University
- Publication Details
- Ecology and evolution, Vol.15(4), e71298
- Publisher
- British Ecological Society and John Wiley & Sons Ltd.
- Number of pages
- 10
- Identifiers
- 991005774133607891
- Copyright
- © 2025 The Author(s).
- Murdoch Affiliation
- School of Agricultural Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.217 Parasitology - Malaria, Toxoplasmosis & Coccidiosis
- 1.217.1890 Coccidiosis
- Web Of Science research areas
- Ecology
- Evolutionary Biology
- ESI research areas
- Environment/Ecology