An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat

K. Wang; S. Islam; J. Ma; M. Anwar; J. Chen; Y. Yan; R. Appels; W. Ma

doi:10.1111/hrd2.00069

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An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat

Journal article

Open access

Peer reviewed

An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat

K. Wang, S. Islam, J. Ma, M. Anwar, J. Chen, Y. Yan, R. Appels and W. Ma

Hereditas, Vol.151(6), pp.196-200

2014

DOI: https://doi.org/10.1111/hrd2.00069

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Abstract

Wheat bread-making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over-expression (Bx7oe), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS-PAGE and normal RP-HPLC procedures failed to separate the two alleles. In the current study, an extensively optimised MALDI-TOF based procedure and a refined DNA based marker for efficiently differentiating Bx7oe from normal Bx7 allele were established. Results indicated that the MALDI-TOF procedure is cost effective, high throughput, and proven reliable, while the refined PCR marker only amplifies Bx7oe allele, a clear advantage over the previously developed codominant marker.

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Title: An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat
Authors/Creators: K. Wang (Author/Creator) - Murdoch University
S. Islam (Author/Creator) - Murdoch University
J. Ma (Author/Creator) - Murdoch University
M. Anwar (Author/Creator) - Murdoch University
J. Chen (Author/Creator) - Chengdu Institute of Biology
Y. Yan (Author/Creator) - Capital Normal University
R. Appels (Author/Creator) - Murdoch University
W. Ma (Author/Creator) - Murdoch University
Publication Details: Hereditas, Vol.151(6), pp.196-200
Publisher: Blackwell Publishing Inc
Identifiers: 991005542450807891
Copyright: © 2015 The Authors
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics