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An improved technique for fluorescence staining of fungal nuclei and septa
Journal article   Peer reviewed

An improved technique for fluorescence staining of fungal nuclei and septa

Y. Hua'an, K. Sivasithamparam and P.A. O'Brien
Australasian Plant Pathology, Vol.20(3), pp.119-121
1991
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Abstract

Nuclei and septa of fungal mycelium grown on glass slides coated with potato-dextrose agar stained very clearly with fluorescent Hoechst Dye 33258 in buffer of pH 7.8 and 10.5, respectively. The technique is simple, rapid, and gives excellent and clear staining.

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