An in vivo and in vitro comparison of the effects of vasoactive mediators on pulpal blood vessels in rat incisors

C.Y. Yu; N.M. Boyd; S.J. Cringle; E.N. Su; V.A. Alder; D.Y. Yu

doi:10.1016/S0003-9969(02)00066-3

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An in vivo and in vitro comparison of the effects of vasoactive mediators on pulpal blood vessels in rat incisors

Journal article

Peer reviewed

An in vivo and in vitro comparison of the effects of vasoactive mediators on pulpal blood vessels in rat incisors

C.Y. Yu, N.M. Boyd, S.J. Cringle, E.N. Su, V.A. Alder and D.Y. Yu

Archives of Oral Biology, Vol.47(10), pp.723-732

2002

DOI: https://doi.org/10.1016/S0003-9969(02)00066-3

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Abstract

The effects of endogenous vasoactive substances were evaluated in anaesthetized rats using a laser Doppler flowmeter to monitor changes in pulpal blood flow, as well as directly in isolated pulpal arteriole preparations utilising a microperfusion and monitoring system to observe changes in vessel diameter. In anaesthetized rats, while systemic arterial blood pressure remained relatively stable, intra-arterial delivery of adrenaline (epinephrine) (A), noradrenaline (norepinephrine) (NA), phenylephrine (PHE), dopamine (DOPA), 5-hydroxytryptamine (5-HT), or endothelin-1 (ET-1) produced a dose-dependent reduction in pulpal blood flow (order of potency: ET-1 ≫ A = NA > PHE = DOPA = 5-HT); acetylcholine induced a dose-dependent increase in pulpal blood flow; histamine, isoproterenol and adenosine produced no significant changes. In isolated arteriole preparations, intraluminal delivery of A, NA, PHE, DOPA or 5-HT produced dose-dependent vasoconstriction (A = NA > PHE = DOPA = 5-HT). Acetylcholine relaxed NA-precontracted vessels dose-dependently. Histamine and isoproterenol produced a small vasodilatation. Intraluminal ET-1 produced a small vasoconstriction at 10-8 M, whereas extraluminal ET-1 produced a dose-dependent vasoconstriction from 10-10 M and above. Intraluminal adenosine failed to dilate vessels precontracted with ET-1, whereas extraluminal adenosine caused a complete relaxation. These combined in vivo and in vitro data suggest that, in the rat incisor, the pulpal microcirculation is capable of functional regulation and that pulpal blood flow may be modulated by endothelium-related factors, metabolic (tissue-related) factors, as well as humoral (blood-borne) factors.

Details

Title: An in vivo and in vitro comparison of the effects of vasoactive mediators on pulpal blood vessels in rat incisors
Authors/Creators: C.Y. Yu (Author/Creator) - The University of Western Australia
N.M. Boyd (Author/Creator) - The University of Western Australia
S.J. Cringle (Author/Creator) - The University of Western Australia
E.N. Su (Author/Creator) - The University of Western Australia
V.A. Alder (Author/Creator) - Murdoch University
D.Y. Yu (Author/Creator) - Murdoch University
Publication Details: Archives of Oral Biology, Vol.47(10), pp.723-732
Publisher: Elsevier
Identifiers: 991005542249107891
Copyright: © 2002 Elsevier Science Ltd.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.195 Neuroendocrine & Intestinal Disorders; 1.195.578 Neuropeptide Roles
Web Of Science research areas: Dentistry, Oral Surgery & Medicine
ESI research areas: Clinical Medicine