Journal article
Analysis of Small RNA Sequencing Data in Plants
Plant Bioinformatics, Vol.2443, pp.497-509
2022
Abstract
Over the past decades, next-generation sequencing (NGS) has been employed extensively for investigating the regulatory mechanisms of small RNAs. Several bioinformatics tools are available for aiding biologists to extract meaningful information from enormous amounts of data generated by NGS platforms. This chapter describes a detailed methodology for analyzing small RNA sequencing data using different open source tools. We elaborate on various steps involved in analysis, from processing the raw sequencing reads to identifying miRNAs, their targets, and differential expression studies.
Details
- Title
- Analysis of Small RNA Sequencing Data in Plants
- Authors/Creators
- V. Garg (Author/Creator)R.K. Varshney (Author/Creator)
- Publication Details
- Plant Bioinformatics, Vol.2443, pp.497-509
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005542376107891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; Food Futures Institute; Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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