Journal article
Analysis of an intraspecific RIL population uncovers genomic segments harbouring multiple QTL for seed relevant traits in lentil (Lens culinaris L.)
Physiology and Molecular Biology of Plants, Vol.23(3), pp.675-684
2017
Abstract
Improving seed related traits remains key objective in lentil breeding. In recent years, genomic resources have shown great promise to accelerate crop improvement. However, limited genomic resources in lentil greatly restrict the use of genomics assisted breeding. The present investigation aims to build an intraspecific genetic linkage map and identify the QTL associated with important seed relevant traits using 94 recombinant inbreds (WA 8649090 × Precoz). A total of 288 polymorphic DNA markers including simple sequence repeat (SSR), inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) were assayed on mapping population. The resultant genetic linkage map comprised 220 loci spanning 604.2 cM of the lentil genome, with average inter-marker distance of 2.74 cM. QTL mapping in this RIL population uncovered a total of 18 QTL encompassing nine major and nine minor QTL. All major QTL were detected for seed related traits viz., seed diameter (SD), seed thickness (ST), seed weight (SW) and seed plumpness (SP) across two locations. A considerable proportion of the phenotypic variation (PV) was accounted to these QTL. For instance, one major QTL on LG5 controlling SW (QTL 15) explained 50% PV in one location, while the same QTL accounted for 34.18% PV in other location. Importantly, the genomic region containing multiple QTL for different seed traits was mapped to a 17-cM region on LG5. The genomic region harbouring QTL for multiple traits opens up exciting opportunities for genomics assisted improvement of lentil.
Details
- Title
- Analysis of an intraspecific RIL population uncovers genomic segments harbouring multiple QTL for seed relevant traits in lentil (Lens culinaris L.)
- Authors/Creators
- R. Jha (Author/Creator) - Chaudhary Sarwan Kumar Himachal Pradesh Krishi VishvavidyalayaA. Bohra (Author/Creator) - Indian Institute of Pulses ResearchU.C. Jha (Author/Creator) - Indian Institute of Pulses ResearchM. Rana (Author/Creator) - Indian Grassland and Fodder Research InstituteR.K. Chahota (Author/Creator) - Chaudhary Sarwan Kumar Himachal Pradesh Krishi VishvavidyalayaS. Kumar (Author/Creator) - International Center for Agricultural Research in the Dry AreasT.R. Sharma (Author/Creator) - Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
- Publication Details
- Physiology and Molecular Biology of Plants, Vol.23(3), pp.675-684
- Publisher
- Springer
- Identifiers
- 991005540848707891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science