Journal article
Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae
Epidemiology and Infection, Vol.103(02), pp.275-284
1989
Abstract
Lipopolysaccharide (LPS) extracts obtained from Treponema hyodysenteriae of serogroups A, B, D and E, and from T. innocens were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit sera. All organisms possessed multiple LPS bands, but their position and number differed.
Immunoblotting of LPS with grouping sera identified three or four major antigenic LPS components in the 10-42 kDa range in all organisms: these components were largely specific to each type-organism of a serogroup, and presumably represented group antigens. Although some minor cross-reactivity occurred between LPS from organisms in the different groups, this was insufficient to merit changes to the current LPS serogrouping system for T. hyodysenteriae. Besides this LPS ‘complex’, other higher-molecular-weight material which appeared to be a common component of the treponemes examined was present in low concentrations. Organisms with different serotypes within a serogroup apparently possessed common LPS bands, but also had unique LPS bands which may account for their serotype specificity. One ‘untypable’ organism lacked group-specific LPS and was thought to be a mutant of a group B organism. The loss of serogroup LPS by the isolate suggested that this material is an external component of the cell wall. The availability of an atypical organism lacking LPS components may facilitate further studies on the pathogenesis of swine dysentery.
Details
- Title
- Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae
- Authors/Creators
- D.J. Hampson (Author/Creator) - Murdoch UniversityJ.R.L. Mhoma (Author/Creator) - Murdoch UniversityB. Combs (Author/Creator) - Murdoch University
- Publication Details
- Epidemiology and Infection, Vol.103(02), pp.275-284
- Publisher
- Cambridge University Press
- Identifiers
- 991005541153307891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
210 File views/ downloads
43 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2445 Lawsonia Intracellularis
- Web Of Science research areas
- Infectious Diseases
- Public, Environmental & Occupational Health
- ESI research areas
- Social Sciences, general