Analysis of multiple Brachyspira hyodysenteriae genomes confirms that the species is relatively conserved but has potentially important strain variation

M. Black; P. Moolhuijzen; R. Barrero; T. La; N. Phillips; D. Hampson; W. Herbst; S. Barth; M. Bellgard

doi:10.1371/journal.pone.0131050

Back

Analysis of multiple Brachyspira hyodysenteriae genomes confirms that the species is relatively conserved but has potentially important strain variation

Journal article

Open access

Peer reviewed

Analysis of multiple Brachyspira hyodysenteriae genomes confirms that the species is relatively conserved but has potentially important strain variation

M. Black, P. Moolhuijzen, R. Barrero, T. La, N. Phillips, D. Hampson, W. Herbst, S. Barth and M. Bellgard

PloS one, Vol.10(6), e0131050

2015

DOI: https://doi.org/10.1371/journal.pone.0131050

Files and links (2)

pdf

multiple_brachyspira.pdfDownload View

Published (Version of Record) Open Access

url

Free to Read *No subscription requiredView

Abstract

The intestinal spirochete Brachyspira hyodysenteriae is an important pathogen in swine, causing mucohemorrhagic colitis in a disease known as swine dysentery. Based on the detection of significant linkage disequilibrium in multilocus sequence data, the species is considered to be clonal. An analysis of the genome sequence of Western Australian B. hyodysenteriae strain WA1 has been published, and in the current study 19 further strains from countries around the world were sequenced with Illumina technology. The genomes were assembled and aligned to over 97.5% of the reference WA1 genome at a percentage sequence identity better than 80%. Strain regions not aligned to the reference ranged between 0.2 and 2.5%. Clustering of the strain genes found on average 2,354 (88%) core genes, 255 (8.6%) ancillary genes and 77 (2.9%) unique genes per strain. Depending on the strain the proportion of genes with 100% sequence identity to WA1 ranged from 85% to 20%. The result is a global comparative genomic analysis of B. hyodysenteriae genomes revealing potential differential phenotypic markers for numerous strains. Despite the differences found, the genomes were less varied than those of the related pathogenic species Brachyspira pilosicoli, and the analysis supports the clonal nature of the species. From this study, a public genome resource has been created that will serve as a repository for further genetic and phenotypic studies of these important porcine bacteria. This is the first intra-species B. hyodysenteriae comparative genomic analysis.

Details

Title: Analysis of multiple Brachyspira hyodysenteriae genomes confirms that the species is relatively conserved but has potentially important strain variation
Authors/Creators: M. Black (Author/Creator) - Murdoch University
P. Moolhuijzen (Author/Creator) - Murdoch University
R. Barrero (Author/Creator) - Murdoch University
T. La (Author/Creator) - Murdoch University
N. Phillips (Author/Creator) - Murdoch University
D. Hampson (Author/Creator) - Murdoch University
W. Herbst (Author/Creator) - Justus-Liebig-Universität Gießen
S. Barth (Author/Creator) - Justus-Liebig-Universität Gießen
M. Bellgard (Author/Creator) - Murdoch University
Publication Details: PloS one, Vol.10(6), e0131050
Publisher: Public Library of Science
Identifiers: 991005540055107891
Copyright: © 2015 Black et al.
Murdoch Affiliation: Centre for Comparative Genomics; School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

123 File views/ downloads

48 Record Views

25 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.2445 Lawsonia Intracellularis
Web Of Science research areas: Microbiology
ESI research areas: Molecular Biology & Genetics