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Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH)
Journal article   Peer reviewed

Analysis of rye B-chromosome structure using fluorescencein situ hybridization (FISH)

T.M. Wilkes, M.G. Francki, P. Langridge, A. Karp, R.N. Jones and J.W. Forster
Chromosome Research, Vol.3(8), pp.466-472
1995
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Abstract

Fluorescencein situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomicin situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A–B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.

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Collaboration types
Domestic collaboration
International collaboration
Citation topics
1 Clinical & Life Sciences
1.186 Chromosome Disorders
1.186.670 Chromosomal Evolution
Web Of Science research areas
Biochemistry & Molecular Biology
Genetics & Heredity
ESI research areas
Molecular Biology & Genetics
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