Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay

S. Duncan; J.E. Barton; P.A. O'Brien

doi:10.1016/S0953-7562(09)80508-X

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Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay

Journal article

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Peer reviewed

Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay

S. Duncan, J.E. Barton and P.A. O'Brien

Mycological Research, Vol.97(9), pp.1075-1082

1993

DOI: https://doi.org/10.1016/S0953-7562(09)80508-X

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Abstract

Genetic variation in Australian isolates of Rhizoctonia solani was analyzed by random amplified polymorphic DNA (RAPD) assay. Isolates originated from different geographic locations in Australia and belong to a number of different anastomosis and pectic zymogram groups. Using four different oligonucleotide primers fingerprint patterns were generated for each isolate. All of the anastomosis and pectic zymogram groups (including subgroups) tested could be distinguished. For some groups there was considerable variation in the fingerprint patterns between isolates. This variation was more marked between isolates from different geographic locations. Other groups showed very little variation between isolates. RAPD-PCR analysis is a very useful alternative to anastomosis grouping for identification of isolates of R. solani.

Details

Title: Analysis of variation in isolates of Rhizoctonia solani by random amplified polymorphic DNA assay
Authors/Creators: S. Duncan (Author/Creator)
J.E. Barton (Author/Creator)
P.A. O'Brien (Author/Creator)
Publication Details: Mycological Research, Vol.97(9), pp.1075-1082
Publisher: Cambridge University Press
Identifiers: 991005544154907891
Copyright: © 1993, British Mycological Society.
Murdoch Affiliation: School of Biological and Environmental Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.556 Microbial Biocontrol
Web Of Science research areas: Mycology
ESI research areas: Plant & Animal Science