Journal article
Anchored PCR cloning of the novel HLA-Cw*0704 allele detected by PCR-SSP
Tissue Antigens, Vol.46(1), pp.19-23
1995
Abstract
The novel HLA-Cw*0704 allele, previously detected as the PCR-SSP variant Cw7/8v, has been cloned and sequenced from the homozygous typing cell KR03/4 after amplification by anchored PCR. The nucleotide sequence of Cw*0704 is closely related to those of other Cw*07 alleles, but carries specific changes in exon 3 consistent with its serological behavior - a short Cw7 cross-reactive with antibodies directed against HLA-Cw8. Some of the substitutions of Cw*0704 have not been previously described for HLA-C but are found in HLA-B alleles and in published C sequences of non-human primates. The new allele carries a novel polymorphism in its 5′ untranslated region (5′ut) that could be shared by all Cw*07 alleles. By PCR-SSP, Cw*0704 is a relatively common allele in English Caucasoids at a frequency of 4.6%. It is most often observed on HLA-B44 haplotypes previously described as HLA-C “blank”, although linkage disequilibria with other HLA-B specificities have been found.
Details
- Title
- Anchored PCR cloning of the novel HLA-Cw*0704 allele detected by PCR-SSP
- Authors/Creators
- C. Vilches (Author/Creator) - Hospital Universitario Puerta de Hierro MajadahondaM. Bunce (Author/Creator) - Tissue Typing Laboratory, Oxford Transplant Centre, Oxford, United KingdomR. Pablo (Author/Creator)M. J. Herrero (Author/Creator) - Hospital Universitario Puerta de Hierro MajadahondaM. Kreisler (Author/Creator) - Hospital Universitario Puerta de Hierro Majadahonda
- Publication Details
- Tissue Antigens, Vol.46(1), pp.19-23
- Publisher
- Blackwell Publishing
- Identifiers
- 991005543382407891
- Copyright
- 1995 Munksgaard
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.607 MHC Diversity
- Web Of Science research areas
- Cell Biology
- Immunology
- Pathology
- ESI research areas
- Molecular Biology & Genetics