Journal article
Ancient DNA extraction from soils and sediments
Ancient DNA, Vol.840, pp.57-63
2012
Abstract
DNA contained in soils and sediments can provide novel insights into past environments and ecosystems. In this chapter, I describe an efficient and effective technique to extract total DNA from sediments in a manner that minimizes the coextraction of PCR-inhibitory compounds. I describe two different approaches: one that is suitable for large (up to 10 g wet weight) amounts of substrate, and a second that is more appropriate for small (up to 0.5 g) amounts of substrate. Finally, I discuss some of the obstacles that may be encountered in the process of extracting DNA from soils and sediments and suggest approaches to circumvent some common problems.
Details
- Title
- Ancient DNA extraction from soils and sediments
- Authors/Creators
- J. Haile (Author/Creator)
- Publication Details
- Ancient DNA, Vol.840, pp.57-63
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005541662107891
- Copyright
- © 2012 Springer Science+Business Media, LLC
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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