Journal article
Anti-β2GP1 antibodies have variable effects on platelet aggregation
Pathology, Vol.45(2), pp.155-161
2013
Abstract
Aim
To investigate the effects of affinity-purified rabbit anti-β2GP1, and anti-β2GP1 purified from patients with systemic lupus erythematosus (SLE), on adenosine diphosphate (ADP)-induced aggregation.
Methods
Whole blood was collected and processed to obtain platelet poor plasma (PPP) from normal controls (n = 15) and SLE patients (n = 15). Using PPP, anti-β2GP1 titres were determined using an ELISA and IgG fractions isolated using a HiTrap protein G column. Anti-β2GP1 was purified from two SLE patients using purified β2GP1 coupled to a HiTrap NHS-activated HP column.
Results
The effect of rabbit and human derived anti-β2GP1 (0–100μg/mL), on ADP (2.5, 5μM) induced platelet aggregation were investigated using light transmission aggregometry. Rabbit anti-β2GP1 significantly inhibited all parameters of 5μM ADP-induced platelet aggregation; %Max (p = 0.028), %AUC (p = 0.014) and slope (p<0.001). In contrast, anti-β2GP1 purified from SLE patients significantly enhanced the %Max (p = 0.031) and %AUC (p = 0.007) in a concentration dependent manner, but inhibited the slope (p<0.05) of 5μM ADP-induced platelet aggregation.
Conclusion
Our data suggest anti-β2GP1 purified from different species have variable effects on in vitro platelet aggregation. The disparity between rabbit and human anti-β2GP1 may be due to the heterogeneous nature of anti-β2GP1, varying avidity or different antibody binding specificities between species.
Details
- Title
- Anti-β2GP1 antibodies have variable effects on platelet aggregation
- Authors/Creators
- N.A. Betts (Author/Creator) - University of TasmaniaK.D.K. Ahuja (Author/Creator) - University of TasmaniaM.J. Adams (Author/Creator) - University of Tasmania
- Publication Details
- Pathology, Vol.45(2), pp.155-161
- Publisher
- Royal College of Pathologists of Australasia
- Identifiers
- 991005543938407891
- Copyright
- © 2013 Royal College of Pathologists of Australasia
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.75 Blood Clotting
- 1.75.326 Antiphospholipid Syndrome
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine