Journal article
Anti-tissue factor pathway inhibitor activity in subjects with antiphospholipid syndrome is associated with increased thrombin generation
Haematologica, Vol.89(8), pp.985-990
2004
Abstract
BACKGROUND AND OBJECTIVES:
Immunoglobulin G (IgG) fractions from subjects with antiphospholipid syndrome (aPS) have previously been demonstrated to have inhibitory activity against tissue factor pathway inhibitor (TFPI). This may contribute to the development of a prothrombotic state by impaired regulation of the tissue factor (TF) pathway. This study investigated the effect that IgG fractions from aPS subjects containing anti-TFPI activity have on in vitro TF-induced thrombin generation.
DESIGN AND METHODS:
TFPI and anti-TFPI activities were determined in normal controls (n=29) and aPS subjects (n=57). TFPI activity was determined using an amidolytic assay based on the generation of factor Xa. Anti-TFPI activity was determined after incubating IgG isolated from a control or subject plasma with pooled normal plasma, using the TFPI activity assay. The influence of IgG fractions from controls (n=10) and subjects (n=23) on TF-induced in vitro thrombin generation was determined using a chromogenic assay of thrombin activity.
RESULTS:
TFPI activity in controls (1.13+/-0.25 U/mL) was significantly lower than in subjects (1.30+/-0.42 U/mL) (p < 0.05). Anti-TFPI activity was significantly higher in subjects than controls (p = 0.0001). TF-induced thrombin generation was positively associated with anti-TFPI activity (r = 0.356; p > 0.05), with increased levels of each demonstrated in 5 subjects.
INTERPRETATION AND CONCLUSIONS:
Anti-TFPI activity was confirmed in 65% of aPS subjects. IgG fractions demonstrated a variable ability to interfere with TFPI function and TF-induced thrombin generation. Cross-reacting antiphospholipid antibodies and/or other entities may interfere with TFPI function, resulting in a net increase in thrombin generation and an increased thrombotic risk.
Details
- Title
- Anti-tissue factor pathway inhibitor activity in subjects with antiphospholipid syndrome is associated with increased thrombin generation
- Authors/Creators
- M. Adams (Author/Creator)L. Breckler (Author/Creator)P. Stevens (Author/Creator)J. Thom (Author/Creator)R. Baker (Author/Creator)R. Oostryck (Author/Creator)
- Publication Details
- Haematologica, Vol.89(8), pp.985-990
- Publisher
- Ferrata Storti Foundation
- Identifiers
- 991005544782907891
- Copyright
- © 2004, Ferrata Storti Foundation
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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