Journal article
Antimicrobial activity of natural products against Clostridium difficile in vitro
Journal of Applied Microbiology, Vol.123(1), pp.92-103
2017
Abstract
Aims: To investigate the antimicrobial activity of various natural products against Clostridium difficile in vitro.
Methods and Results: The antibacterial activity of 20 natural products was determined by the agar well diffusion and broth microdilution assays against four C. difficile strains, three comparator organisms and four gastrointestinal commensal organisms. Of the raw natural products, garlic juice had the highest activity. The most active processed products were peppermint oil and the four pure compounds trans-cinnamaldehyde, allicin, menthol and zingerone. Furthermore, Bacteroides species had similar susceptibility to C. difficile to most natural products; however, Lactobacillus casei was less susceptible. The combined effect of natural products with vancomycin or metronidazole was determined using the conventional checkerboard titration method and the fractional inhibitory concentration index was calculated. The results showed a possible synergism between trans-cinnamaldehyde and vancomycin and partial synergy between trans-cinnamaldehyde and metronidazole.
Conclusions: The study indicates a range of antimicrobial activity of natural products against C. difficile and suggests that they may be useful as alternative or complementary treatments for C. difficile infection (CDI), particularly as most are able to be given orally. Significance and Impact of the Study: This study encourages further investigation of natural products for treatment of CDI.
Details
- Title
- Antimicrobial activity of natural products against Clostridium difficile in vitro
- Authors/Creators
- N. Roshan (Author/Creator) - The University of Western AustraliaT.V. Riley (Author/Creator) - The University of Western AustraliaK.A. Hammer (Author/Creator) - The University of Western Australia
- Publication Details
- Journal of Applied Microbiology, Vol.123(1), pp.92-103
- Publisher
- Blackwell Publishing
- Identifiers
- 991005543639107891
- Copyright
- © Society for Applied Microbiology
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.1133 Clostridium Infections
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
- ESI research areas
- Biology & Biochemistry