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CC BY V4.0, Open Access
Journal article
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
Frontiers in plant science, Vol.14, Art. 1157678
2023
Abstract
Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches and transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery of engineered nucleases as genetic scissors to carry out precise manipulation in crop stress-responsive genes and associated molecular network has paved the way for sustainable management of abiotic stress conditions. In this context, the clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool has revolutionized due to its simplicity, accessibility, adaptability, flexibility, and wide applicability. This system has great potential to build up crop varieties with enhanced tolerance against abiotic stresses. In this review, we summarize the latest findings on understanding the mechanism of abiotic stress response in plants and the application of CRISPR/Cas-mediated gene-editing system towards enhanced tolerance to a multitude of stresses including drought, salinity, cold, heat, and heavy metals. We provide mechanistic insights on the CRISPR/Cas9-based genome editing technology. We also discuss applications of evolving genome editing techniques such as prime editing and base editing, mutant library production, transgene free and multiplexing to rapidly deliver modern crop cultivars adapted to abiotic stress conditions.
Details
- Title
- Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants
- Authors/Creators
- Manoj Kumar - , , , , , , , , , , , ,Manas Ranjan Prusty - , , , , , , , , , , , ,Manish K. Pandey - , , , , , , , , , , , ,Prashant Kumar Singh - , , , , , , , , , , , ,Abhishek Bohra - , , , , , , , , , , , ,Baozhu Guo - Yangzhou UniversityRajeev K. Varshney - Murdoch University, Food Futures Institute
- Publication Details
- Frontiers in plant science, Vol.14, Art. 1157678
- Publisher
- Frontiers Media S.A
- Identifiers
- 991005571669307891
- Copyright
- © 2023 Kumar et al.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1401 Genome Editing
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science