Journal article
Application of laser microdissection to study plant-fungal pathogen interactions
Molecular and Cell Biology Methods for Fungi, Vol.638, pp.153-163
2010
Abstract
Laser microdissection (LM) has become an important tool for isolating individual cells or cell types from suitably prepared tissue samples. The technique can be used to isolate both fungal and host plant cells after pathogen infection for molecular studies. Sample preparation is a crucial step in LM and involves fixing samples with appropriate fixatives to preserve the integrity of cell morphology and target metabolites (e.g., RNA), and embedding the fixed tissue in paraffin wax for sectioning onto microscope slides. The sample sections are then deparaffinised, rehydrated, and cells are dissected by a laser focused through a microscope. LM samples are collected into protective (e.g., RNAse-free) medium for isolation of RNA. The RNA can then be subjected to gene expression studies such as quantitative RT-PCR and microarray analysis after a linear RNA amplification process.
Details
- Title
- Application of laser microdissection to study plant-fungal pathogen interactions
- Authors/Creators
- J. Fosu-Nyarko (Author/Creator) - School of Biological Sciences and Biotechnology Murdoch UniversityM.G.K. Jones (Author/Creator)Z. Wang (Author/Creator) - School of Biological Sciences and Biotechnology Murdoch University
- Publication Details
- Molecular and Cell Biology Methods for Fungi, Vol.638, pp.153-163
- Publisher
- Humana Press, Inc.
- Identifiers
- 991005541493807891
- Copyright
- © Springer Science+Business Media, LLC 2010
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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