Journal article
Application of nanoscale secondary ion mass spectrometry to plant cell research
Plant signaling & behavior, Vol.5(6), pp.760-762
2010
PMCID: PMC3001583
PMID: 20418660
Abstract
Imaging resource flow in soil-plant systems remains central to understanding plant development and interactions with the environment. Typically, subcellular resolution is required to fully elucidate the compartmentation, behavior, and mode of action of organic compounds and mineral elements within plants. For many situations this has been limited by the poor spatial resolution of imaging techniques and the inability to undertake studies in situ. Here we demonstrate the potential of Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS), which is capable of the quantitative high-resolution spatial imaging of stable isotopes (e. g., C-12, C-13, N-14, N-15, O-16, O-18, P-31, S-34) within intact plant-microbial-soil systems. We present examples showing how the approach can be used to investigate competition for N-15-labelled nitrogen compounds between plant roots and soil microorganisms living in the rhizosphere and the spatial imaging of P-31 in roots. We conclude that NanoSIMS has great potential to elucidate the flow of isotopically-labelled compounds in complex media (e.g., soil) and opens up countless new opportunities for studying plant responses to abiotic stress (e. g., O-18(3), elevated (CO2)-C-13), signal exchange, nutrient flow and plant-microbial interactions.
Details
- Title
- Application of nanoscale secondary ion mass spectrometry to plant cell research
- Authors/Creators
- Matt R. Kilburn - The University of Western AustraliaDavid L. Jones - Bangor UniversityPeta L. Clode - The University of Western AustraliaJohn B. Cliff - The University of Western AustraliaElizabeth A. Stockdale - Newcastle UniversityAnke M. Herrmann - University of Newcastle AustraliaDaniel V. Murphy - The University of Western Australia
- Publication Details
- Plant signaling & behavior, Vol.5(6), pp.760-762
- Publisher
- Taylor & Francis
- Number of pages
- 3
- Grant note
- DP0985832 / ARC Discovery Project; Australian Research Council Grains Research and Development Corporation (GRDC); Grains R&D Corp
- Identifiers
- 991005560323907891
- Copyright
- © 2010 Landes Bioscience
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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