Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

D. McGill; E. Chekmeneva; J.C. Lindon; Z. Takats; J.K. Nicholson

doi:10.1039/c8fd00220g

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Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

Journal article

Open access

Peer reviewed

Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine

D. McGill, E. Chekmeneva, J.C. Lindon, Z. Takats and J.K. Nicholson

Faraday Discussions, Vol.218, pp.395-416

2019

DOI: https://doi.org/10.1039/c8fd00220g

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Abstract

Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artificial urine mixtures, which produce unique retention profiles useful for metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.

Details

Title: Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine
Authors/Creators: D. McGill (Author/Creator) - Imperial College London
E. Chekmeneva (Author/Creator) - Imperial College London
J.C. Lindon (Author/Creator) - Imperial College London
Z. Takats (Author/Creator) - Imperial College London
J.K. Nicholson (Author/Creator) - Murdoch University
Publication Details: Faraday Discussions, Vol.218, pp.395-416
Publisher: Royal Society of Chemistry
Identifiers: 991005543822107891
Murdoch Affiliation: Australian National Phenome Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.211 Mass Spectrometry; 2.211.990 Metabolomics
Web Of Science research areas: Chemistry, Physical
ESI research areas: Chemistry