Journal article
Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
Scientific Reports, Vol.7(1)
2017
Abstract
The development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report, we show that EpCAM-aptamer-guided survivin RNAi effectively downregulated survivin both in colorectal cancer cells in vitro and in a mouse xenograft model for colorectal cancer. When combined with the conventional chemotherapeutic agents, the aptamer-guided survivin RNAi was able to enhance the sensitivity towards 5-FU or oxaliplatin in colorectal cancer stem cells, increase apoptosis, inhibit tumour growth and improve the overall survival of mice bearing xenograft colorectal cancer. Our results indicate that survivin is one of the key players responsible for the innate chemoresistance of colorectal cancer stem cells. Thus, aptamer-mediated targeting of survivin in cancer stem cells in combination with chemotherapeutic drugs constitutes a new avenue to improve treatment outcome in oncologic clinics.
Details
- Title
- Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
- Authors/Creators
- H. Al Shamaileh (Author/Creator)T. Wang (Author/Creator) - Deakin UniversityD. Xiang (Author/Creator) - Deakin UniversityW. Yin (Author/Creator) - Deakin UniversityP.H-L Tran (Author/Creator) - Deakin UniversityR.A. Barrero (Author/Creator) - Murdoch UniversityP-Z Zhang (Author/Creator) - Suzhou GenePharma, 199 Dongping Street, Suzhou, 215123, China.Y. Li (Author/Creator) - St George HospitalL. Kong (Author/Creator) - Deakin UniversityK. Liu (Author/Creator) - Sichuan UniversityS-F Zhou (Author/Creator) - Jimei UniversityY. Hou (Author/Creator) - Shaanxi Normal UniversityS. Shigdar (Author/Creator) - Deakin UniversityW. Duan (Author/Creator) - Deakin University
- Publication Details
- Scientific Reports, Vol.7(1)
- Publisher
- Springer Nature
- Identifiers
- 991005543772407891
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.108 Molecular & Cell Biology - Cancer & Development
- 1.108.591 Cancer Stem Cells
- Web Of Science research areas
- Oncology
- ESI research areas
- Clinical Medicine