Journal article
Are Sebacinaceae common and widespread ectomycorrhizal associates of Eucalyptus species in Australian forests?
Mycorrhiza, Vol.12(5), pp.243-247
2002
Abstract
A molecular survey of basidiomycete ectomycorrhizal fungi colonising root tips at a site in Eucalyptus marginata (jarrah) forest revealed the presence of many fungal species which could not be identified from a database of ITS-PCR-RFLP profiles from morphologically identified species. Three of these unidentified taxa were among the six most frequently encountered profiles. Phylogenetic analyses of ITS and nuclear LSU sequences revealed a close relationship among the three fungi and that they belong to the family Sebacinaceae (sensu Weiß and Oberwinkler 2001). The possibility that DNA of non-ectomycorrhizal rhizosphere or endophytic fungi had been amplified selectively by the basidiomycete-specific primers was tested by amplification with fungal-specific primers. A single PCR fragment was amplified in all but two of the 24 samples tested and digestion with two restriction enzymes produced RFLP profiles which matched those from the Sebacinoid sequence. We conclude, therefore, that at least three species of Sebacinaceae are common ectomycorrhizal associates of E. marginata.
Details
- Title
- Are Sebacinaceae common and widespread ectomycorrhizal associates of Eucalyptus species in Australian forests?
- Authors/Creators
- M. Glen (Author/Creator)I.C. Tommerup (Author/Creator)N.L. Bougher (Author/Creator)P.A. O'Brien (Author/Creator)
- Publication Details
- Mycorrhiza, Vol.12(5), pp.243-247
- Publisher
- Springer Verlag
- Identifiers
- 991005540473907891
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.488 Mycorrhizal Symbiosis
- Web Of Science research areas
- Mycology
- Plant Sciences
- ESI research areas
- Plant & Animal Science